ABM-27132
Anti-Alkaline Phosphatase/ALPL Antibody Picoband®
ABMIUM Verified™|Collaborator Tested|Boster Bio
Primary Antibody supplied by Boster Bio Read more ›
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Product Description
Boster Bio Anti-Alkaline Phosphatase/ALPL Antibody catalog # PA1004-1. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Alkaline phosphatase, tissue-nonspecific isozyme is an enzyme that in humans is encoded by the ALPL gene. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. Liver/bone/kidney alkaline phosphatase, also known as tissue-nonspecific alkaline phosphatase, acts physiologically as a lipid-anchored phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP) ectophosphatase Mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked ly to a disorder known as hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | PA1004-1 |
| Product category | Primary Antibodies |
| Reactive species | Mouse, Rat |
| Tested applications | ELISA, Flow Cytometry, IHC, IHC-P, WB |
| Available conjugations | Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647 |
| Gene name | ALPL |
| Protein name | Alkaline phosphatase, tissue-nonspecific isozyme |
| Specificity | No cross reactivity with other proteins. |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of rat Alkaline Phosphatase, different from the related mouse sequence by one amino acid. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Purification | Immunogen affinity purified. |
| Storage | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| UniProt | P08289 |
| NCBI Gene ID | P08289 |
Available variants
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μg/vial |
| Biotin | 100 μg/vial |
| Cy3 | 100 μg/vial |
| Fluoro488 | 100 μg/vial |
| Fluoro550 | 100 μg/vial |
| Fluoro594 | 100 μg/vial |
| FITC | 100 μg/vial |
| HRP | 100 μg/vial |
| APC | 100 μg/vial |
| PE | 100 μg/vial |
| Fluoro647 | 100 μg/vial |
Key Facts
Product type
Primary Antibody›
Target: Alkaline phosphatase, tissue-nonspecific isozyme
| Target | Alkaline phosphatase, tissue-nonspecific isozyme |
| Also known as | Akp2 antibody; Alkaline phosphatase intestinal antibody; Alkaline phosphatase intestinal precursor antibody; Alkaline phosphatase liver/bone/kidney antibody; Alkaline phosphatase placental antibody; Alkaline phosphatase placental like 2 antibody; Alkaline phosphatase placental like antibody; Alkaline phosphatase placental type antibody; Alkaline phosphomonoesterase antibody; ALP 1 antibody; ALP antibody; ALP I antibody; ALP L antibody; ALP P antibody; ALPG antibody; ALPI antibody; ALPL antibody; ALPP antibody; ALPPL antibody; ALPPL2 antibody; AP TNAP antibody; FLJ40094 antibody; GCAP antibody; Germ cell alkaline phosphatase antibody; Glycerophosphatase antibody; HOPS antibody; IAP antibody; Intestinal alkaline phosphatase antibody; Kasahara isozyme antibody; Nagao isozyme antibody; OTTHUMP00000164357 antibody; Placental alkaline phosphatase antibody; Placental like alkaline phosphatase antibody; PLAP 1 antibody; PLAP antibody; PLAP like antibody; Regan isozyme antibody; Testicular and thymus alkaline phosphatase antibody; Tissue non specific alkaline phosphatase antibody; Tissue nonspecific ALP antibody; TNAP antibody; TNSALP antibody |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Species reactivity | Mouse,Rat |
| Observed band | 57 kDa |
| Purity | No cross reactivity with other proteins. |
| Form / buffer | Lyophilized |
| Shelf life | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647 |
| Non-cross-reactive with | No cross-reactivity with other proteins. |
| Tested applications |
ELISAFlow CytometryIHCIHC-PWB |
| Dilution range | Western blot, 0.1-0.5μg/ml, Rat, Mouse |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of rat Alkaline Phosphatase, different from the related mouse sequence by one amino acid. |
| Purification | Immunogen affinity purified. |
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Reactivity & Application Validation
2 speciess| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Mouse | ||||||||
| Rat |
Specifications
Target
Alkaline phosphatase, tissue-nonspecific isozyme
Also known as
Akp2 antibody; Alkaline phosphatase intestinal antibody; Alkaline phosphatase intestinal precursor antibody; Alkaline phosphatase liver/bone/kidney antibody; Alkaline phosphatase placental antibody; Alkaline phosphatase placental like 2 antibody; Alkaline phosphatase placental like antibody; Alkaline phosphatase placental type antibody; Alkaline phosphomonoesterase antibody; ALP 1 antibody; ALP antibody; ALP I antibody; ALP L antibody; ALP P antibody; ALPG antibody; ALPI antibody; ALPL antibody; ALPP antibody; ALPPL antibody; ALPPL2 antibody; AP TNAP antibody; FLJ40094 antibody; GCAP antibody; Germ cell alkaline phosphatase antibody; Glycerophosphatase antibody; HOPS antibody; IAP antibody; Intestinal alkaline phosphatase antibody; Kasahara isozyme antibody; Nagao isozyme antibody; OTTHUMP00000164357 antibody; Placental alkaline phosphatase antibody; Placental like alkaline phosphatase antibody; PLAP 1 antibody; PLAP antibody; PLAP like antibody; Regan isozyme antibody; Testicular and thymus alkaline phosphatase antibody; Tissue non specific alkaline phosphatase antibody; Tissue nonspecific ALP antibody; TNAP antibody; TNSALP antibody
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Mouse,Rat
Tested applications
ELISAFlow CytometryIHCIHC-PWB
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of rat Alkaline Phosphatase, different from the related mouse sequence by one amino acid.
Observed band
57 kDa
Dilution range
Western blot, 0.1-0.5μg/ml, Rat, Mouse
Purification
Immunogen affinity purified.
Storage & Stability
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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