Collaborator Boster Bio
Primary AntibodiesABMIUM Verified™Collaborator Tested
ABM-23891

Anti-Alanine Transaminase/Gpt Antibody Picoband®

ABMIUM Verified™|Collaborator Tested|Boster Bio

Primary Antibody supplied by Boster Bio Read more ›

Anti-Alanine Transaminase/Gpt Antibody Picoband® Boster Bio
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Anti-Alanine Transaminase/Gpt Antibody Picoband® Boster Bio

Product Description

Anti-Alanine Transaminase/Gpt Antibody Picoband® is a research-use antibody from Boster Bio for studying Alanine aminotransferase 1 in ELISA, Flow Cytometry, IF/ICC, IHC, IHC-P, WB workflows with listed reactivity for Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-Alanine Transaminase/Gpt Antibody Picoband® catalog # A01638-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase

Product information

Key Facts

Product typePrimary Antibodies Target: Alanine aminotransferase 1
Collaborator Boster Bio
Partner model A01638-1
Target Alanine aminotransferase 1
Reactivity Mouse,Rat
Applications ELISA,Flow Cytometry,IF/ICC,IHC,IHC-P,WB
ConjugationUnconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Cross-reactivityNo cross-reactivity with other proteins.
Dilution rangeWestern blot, 0.1-0.25 μg/ml, Mouse, Rat<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Mouse<br> Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Mouse<br> ELISA, 0.1-0.5 μg/ml, -<br>
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Immunogen E.coli-derived mouse Alanine Transaminase/Gpt recombinant protein (Position: A2-S496).
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purification Immunogen affinity purified.
Shelf life At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
AvailabilityAvailable
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Mouse
Rat

Specifications

Target
Alanine aminotransferase 1
Also known as
AAT1; Alanine aminotransferase 1; ALAT1; ALT1; GPT; GPT 1; GPT/ ALT1; GPT1
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Mouse,Rat
Tested applications
ELISAFlow CytometryIF/ICCIHCIHC-PWB
Immunogen
E.coli-derived mouse Alanine Transaminase/Gpt recombinant protein (Position: A2-S496).
Observed band
55 kDa
Dilution range
Western blot, 0.1-0.25 μg/ml, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
ELISA, 0.1-0.5 μg/ml, -
Purification
Immunogen affinity purified.

Storage & Stability

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.
Storage buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Shelf life
At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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