Acetyl Lys proteins rabbit pAb from ELK Biotechnology Read more ›
Acetyl Lys proteins rabbit pAb is a research-use antibody from ELK Biotechnology for studying Acetyl Lys proteins in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey, Plant. Specifications and supporting documents below should be reviewed when planning the experiment.
Acetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs). Signaling resulting in acetylation/deacetylation of histones, transcription factors, and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation, and apoptosis. Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of posttranslational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization, and nuclear transport. The regulation of protein acetylation status is impaired in cancer and polyglutamine diseases, and HDACs have become promising targets for anti-cancer drugs currently in development.
| Specificity | Acetyl-Lys proteins Polyclonal Antibody detects endogenous levels of acetylated Lys proteins. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of HeLa cells, using Lys-Acetylated Proteins Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Lys-Acetylated Proteins Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from COS7 cells treated with TSA 400uM 24h, using Lys-Acetylated Proteins Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from 1) Hela,2)mouse brain, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4384 |
| Target | Acetyl Lys proteins |
| Research areas | Antibody Research |
| Reactivity | Human,Mouse,Rat,Monkey,Plant |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized acetyl-peptide derived from human acetylation Lys proteins. |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Monkey | |||||||
| plant |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Monkey | — | — |
| plant | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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