ACAT-1 rabbit pAb from ELK Biotechnology Read more ›
ACAT-1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ACAT-1 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009],
| Specificity | ACAT-1 Polyclonal Antibody detects endogenous levels of ACAT-1 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using ACAT-1 Polyclonal Antibody | Western Blot analysis of A549 cells using ACAT-1 Polyclonal Antibody | Western blot analysis of lysates from HepG2, Jurkat, 293, and A549 cells, using ACAT1 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HepG2 cells using ACAT1 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1577 |
| Target | ACAT-1 |
| Research areas | >>Fatty acid degradation, >>Valine, leucine and isoleucine degradation, >>Lysine degradation, >>Tryptophan metabolism, >>Pyruvate metabolism, >>Glyoxylate and dicarboxylate metabolism, >>Butanoate metabolism, >>Terpenoid backbone biosynthesis, >>Metabolic pathways, >>Carbon metabolism, >>Fatty acid metabolism, >>Fat digestion and absorption |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ACAT1. AA range:221-270 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Adipocyte,Brain,Fetal brain cortex, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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