ABHD7 rabbit pAb from ELK Biotechnology Read more ›
ABHD7 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ABHD7 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
The α/β hydrolase superfamily comprise diverse members that are involved in important biochemical processes and related to various diseases. They have unrelated sequences, various substrates, and different kinds of catalytic activities, yet they share the same canonical α/β hydrolase fold, which consists of an eight-stranded parallel α/β structure. They are also characterized by a catalytic triad composed of a histidine, an acid and a nucleophile. Members of this superfamily are often drug targets for treating diseases, such as diabetes, Alzheimer's disease, obesity and blood clotting disorders. The Ab hydrolase domain containing (ABHD) gene subfamily is comprised of 15 mostly uncharacterized members, most of which utilize a serine nucleophile to form the G-X-S-X-G nucleophile elbow. ABHD7 is a 362 amino acid single-pass type II membrane protein that belongs to the AB hydrolase superfamily and the Epoxide hydrolase family.
| Specificity | ABHD7 Polyclonal Antibody detects endogenous levels of ABHD7 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using ABHD7 Polyclonal Antibody diluted at 1:500 | Western blot analysis of lysates from HT-29 cells, using ABHD7 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HeLa cells using ABHD7 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1574 |
| Target | ABHD7 |
| Research areas | >>Chemical carcinogenesis - receptor activation, >>Chemical carcinogenesis - reactive oxygen species |
| Reactivity | Human,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ABHD7. AA range:101-150 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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