Acetyl eIF5A/eIF5A2 (K47) rabbit pAb from ELK Biotechnology Read more ›
Acetyl eIF5A/eIF5A2 (K47) rabbit pAb is a research-use antibody from ELK Biotechnology for studying eIF5A2 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
function:The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond.,PTM:eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).,similarity:Belongs to the eIF-5A family.,tissue specificity:Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells.,
| Specificity | Acetyl-eIF5A/eIF5A2 (K47) Polyclonal Antibody detects endogenous levels of eIF5A/eIF5A2 protein only when acetylated at K47. |
|---|---|
| Validation evidence captions | Western blot analysis of L929 lysis using antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysate from L929 cells, using eIF5A (Acetyl-Lys47) Antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1096 |
| Target | eIF5A2 |
| Research areas | Antibody Research |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized Acetyl-peptide derived from human eIF5A around the Acetylation site of Lys47. AA range:11-60 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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