Collaborator ELK Biotechnology
ELISA KitsISO 9001|ABMIUM Verified™
ABM57179

Mouse TAT(Thrombin/Antithrombin Complex) ELISA Kit

ISO 9001|ABMIUM Verified™|ELISA

Mouse TAT(Thrombin/Antithrombin Complex) ELISA Kit from ELK Biotechnology Read more ›

Mouse TAT(Thrombin/Antithrombin Complex) ELISA Kit
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Mouse TAT(Thrombin/Antithrombin Complex) ELISA Kit

Product Description

Mouse TAT(Thrombin/Antithrombin Complex) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring TAT in Plasma samples from Mouse. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TAT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TAT in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include Thrombin/Antithrombin Complex.

Key Facts

Product typeELISA Kits Target: TAT
Collaborator ELK Biotechnology
Partner model ELK1555
Target TAT
Research areasSignal transduction, Enzyme & Kinase, Metabolic pathway, Hematology
Reactivity Mouse
Applications ELISA
Assay type Sandwich
Sample types Plasma
Detection range 0.16-10 ng/mL
Sensitivity 0.059 ng/mL
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Sample Type Validation

1 sample type
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
Sample TypeSERUMPLASMA (EDTA)URINECSFCELL SUPERNATANT
Sample compatibility

Specifications

Target
TAT
Also known as
Thrombin/Antithrombin Complex
Form / buffer
Pre-coated microplate kit
Assay type
Sandwich
Detection method
Colorimetric (TMB/HRP)
Sensitivity
0.059 ng/mL
Detection range
0.16-10 ng/mL
Sample types
Plasma
Plate format
96-well pre-coated
Species reactivity
Mouse

Storage & Stability

Long-term storage
Store according to the conditions stated in the product manual.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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