Human HSPb6(Heat Shock Protein Beta 6) ELISA Kit

Human HSPb6(Heat Shock Protein Beta 6) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring HSPb6 in Tissue homogenates, Cell lysates, Other biological fluids samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSPb6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSPb6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSPb6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSPb6 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include HSP20; Heat Shock Protein 20 kD; Heat Shock Protein B6, Alpha Crystallin Related; Heat shock 20 kDa-like protein p20.