Cattle VB3 (Vitamin B3) ELISA Kit from ELK Biotechnology Read more ›
Cattle VB3 (Vitamin B3) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring VB3 in Serum, Plasma, Other biological fluids samples from Cattle. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle VB3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle VB3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle VB3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative names include VitB3,VB3.
| Collaborator | ELK Biotechnology |
| Partner model | ELK11316 |
| Target | VB3 |
| Research areas | Metabolic pathway, Reproductive science, Nutrition metabolism, Bone metabolism |
| Reactivity | Cattle |
| Applications | ELISA |
| Assay type | Competitive Inhibition |
| Sample types | Serum,Plasma,Other biological fluids |
| Detection range | 3.13-200 ng/mL |
| Sensitivity | 0.93 ng/mL |
| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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