Collaborator ELK Biotechnology
ELISA KitsISO 9001|ABMIUM Verified™
ABM55298

Human VB6(Vitamin B6) ELISA Kit

ISO 9001|ABMIUM Verified™|ELISA

Human VB6(Vitamin B6) ELISA Kit from ELK Biotechnology Read more ›

Human VB6(Vitamin B6) ELISA Kit
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Human VB6(Vitamin B6) ELISA Kit

Product Description

Human VB6(Vitamin B6) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring VB6 in Serum, Plasma, Other biological fluids samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human VB6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VB6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VB6 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include Pyridoxine.

Key Facts

Product typeELISA Kits Target: VB6
Collaborator ELK Biotechnology
Partner model ELK11228
Target VB6
Research areasNutrition metabolism, Hormone metabolism
Reactivity Human
Applications ELISA
Assay type Competitive Inhibition
Sample types Serum,Plasma,Other biological fluids
Detection range 4.69-300 ng/mL
Sensitivity 1.41 ng/mL
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Sample Type Validation

1 sample type
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
Sample TypeSERUMPLASMA (EDTA)URINECSFCELL SUPERNATANT
Sample compatibility

Specifications

Target
VB6
Also known as
Pyridoxine
Form / buffer
Pre-coated microplate kit
Assay type
Competitive Inhibition
Detection method
Colorimetric (TMB/HRP)
Sensitivity
1.41 ng/mL
Detection range
4.69-300 ng/mL
Sample types
SerumPlasmaOther biological fluids
Plate format
96-well pre-coated
Species reactivity
Human

Storage & Stability

Long-term storage
Store according to the conditions stated in the product manual.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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