Horse HB(Hemoglobin) ELISA Kit from ELK Biotechnology Read more ›
Horse HB(Hemoglobin) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring Horse HB in Serum, Plasma, Erythrocyte lysates samples from Horse. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse HB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse HB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse HB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse HB in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative names include Hgb; Haemoglobin; Heterotetramer(αβ)2.
| Collaborator | ELK Biotechnology |
| Partner model | ELK11072 |
| Target | Horse HB |
| Research areas | Metabolic pathway, Infection immunity, Hematology, Neuro science |
| Reactivity | Horse |
| Applications | ELISA |
| Assay type | Sandwich |
| Sample types | Serum,Plasma,Erythrocyte lysates |
| Detection range | 1.56-100 µg/mL |
| Sensitivity | 0.67 µg/mL |
| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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