Collaborator ELK Biotechnology
ELISA KitsISO 9001|ABMIUM Verified™
ABM54171

Chicken HA(Hyaluronic Acid) ELISA Kit

ISO 9001|ABMIUM Verified™|ELISA

Chicken HA(Hyaluronic Acid) ELISA Kit from ELK Biotechnology Read more ›

Chicken HA(Hyaluronic Acid) ELISA Kit
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Chicken HA(Hyaluronic Acid) ELISA Kit

Product Description

Chicken HA(Hyaluronic Acid) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring HA in Serum, Plasma, Other biological fluids samples from Chicken. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken HA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken HA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken HA in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include Hyaluronan; Hyaluronate.

Key Facts

Product typeELISA Kits Target: HA
Collaborator ELK Biotechnology
Partner model ELK10922
Target HA
Research areasTumor immunity, Hepatology
Reactivity Chicken
Applications ELISA
Assay type Competitive Inhibition
Sample types Serum,Plasma,Other biological fluids
Detection range 15.63-1000 ng/mL
Sensitivity 5.12 ng/mL
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Sample Type Validation

1 sample type
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
Sample TypeSERUMPLASMA (EDTA)URINECSFCELL SUPERNATANT
Sample compatibility

Specifications

Target
HA
Also known as
Hyaluronan; Hyaluronate
Form / buffer
Pre-coated microplate kit
Assay type
Competitive Inhibition
Detection method
Colorimetric (TMB/HRP)
Sensitivity
5.12 ng/mL
Detection range
15.63-1000 ng/mL
Sample types
SerumPlasmaOther biological fluids
Plate format
96-well pre-coated
Species reactivity
Chicken

Storage & Stability

Long-term storage
Store according to the conditions stated in the product manual.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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