Collaborator ELK Biotechnology
ELISA KitsISO 9001|ABMIUM Verified™
ABM53337

Cattle IGFBP4(Insulin Like Growth Factor Binding Protein 4) ELISA Kit

ISO 9001|ABMIUM Verified™|ELISA

Cattle IGFBP4(Insulin Like Growth Factor Binding Protein 4) ELISA Kit from ELK Biotechnology Read more ›

Cattle IGFBP4(Insulin Like Growth Factor Binding Protein 4) ELISA Kit
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Cattle IGFBP4(Insulin Like Growth Factor Binding Protein 4) ELISA Kit

Product Description

Cattle IGFBP4(Insulin Like Growth Factor Binding Protein 4) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring IGFBP4 in Serum, Plasma, Other biological fluids samples from Cattle. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle IGFBP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IGFBP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IGFBP4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include BP4; HT29-IGFBP; IBP4.

Key Facts

Product typeELISA Kits Target: IGFBP4
Collaborator ELK Biotechnology
Partner model ELK10693
Target IGFBP4
Research areasApoptosis, Tumor immunity
Reactivity Cattle
Applications ELISA
Assay type Competitive Inhibition
Sample types Serum,Plasma,Other biological fluids
Detection range 1.57-100 ng/mL
Sensitivity 0.56 ng/mL
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Sample Type Validation

1 sample type
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
Sample TypeSERUMPLASMA (EDTA)URINECSFCELL SUPERNATANT
Sample compatibility

Specifications

Target
IGFBP4
Also known as
BP4; HT29-IGFBP; IBP4
Form / buffer
Pre-coated microplate kit
Assay type
Competitive Inhibition
Detection method
Colorimetric (TMB/HRP)
Sensitivity
0.56 ng/mL
Detection range
1.57-100 ng/mL
Sample types
SerumPlasmaOther biological fluids
Plate format
96-well pre-coated
Species reactivity
Cattle

Storage & Stability

Long-term storage
Store according to the conditions stated in the product manual.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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