Mouse C4a(Complement Component 4a) Microsample ELISA Kit

Mouse C4a(Complement Component 4a) Microsample ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring C4a in Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernatant, Other biological fluids samples from Mouse. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse C4a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse C4a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse C4a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse C4a in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include C4a anaphylatoxin.