Collaborator ELK Biotechnology
ELISA KitsISO 9001|ABMIUM Verified™
ABM51275

EasyStep Rat NPT(Neopterin) ELISA Kit

ISO 9001|ABMIUM Verified™|ELISA

EasyStep Rat NPT(Neopterin) ELISA Kit from ELK Biotechnology Read more ›

EasyStep Rat NPT(Neopterin) ELISA Kit
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EasyStep Rat NPT(Neopterin) ELISA Kit

Product Description

EasyStep Rat NPT(Neopterin) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring EasyStep Rat NPT in Serum, Plasma samples from Rat. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NPT antigen, and the Rat NPT standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat NPT to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat NPT in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include Neopterin.

Key Facts

Product typeELISA Kits Target: EasyStep Rat NPT
Collaborator ELK Biotechnology
Partner model ELK101ES
Target EasyStep Rat NPT
Research areasCytokine, Infection immunity
Reactivity Rat
Applications ELISA
Assay type Competitive Inhibition
Sample types Serum,Plasma
Detection range 0.469-30 nmol/L
Sensitivity 0.4 nmol/L
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Sample Type Validation

1 sample type
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
Sample TypeSERUMPLASMA (EDTA)URINECSFCELL SUPERNATANT
Sample compatibility

Specifications

Target
EasyStep Rat NPT
Also known as
Neopterin
Form / buffer
Pre-coated microplate kit
Assay type
Competitive Inhibition
Detection method
Colorimetric (TMB/HRP)
Sensitivity
0.4 nmol/L
Detection range
0.469-30 nmol/L
Sample types
SerumPlasma
Plate format
96-well pre-coated
Species reactivity
Rat

Storage & Stability

Long-term storage
Store according to the conditions stated in the product manual.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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