Human SMARCA4(Transcription activator BRG1) ELISA Kit

Human SMARCA4(Transcription activator BRG1) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring SMARCA4 in Serum, Plasma, Tissue homogenates, Cell lysates samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SMARCA4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SMARCA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SMARCA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SMARCA4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include ATP-dependent helicase SMARCA4;BRG1-associated factor 190A (BAF190A);Mitotic growth and transcription activator;Protein BRG-1;Protein brahma homolog 1;SNF2-beta;SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4.