EasyStep Human IL8(Interleukin 8) ELISA Kit

EasyStep Human IL8(Interleukin 8) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring EasyStep Human IL8 in Cell culture supernatant samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL8, and the Human IL8 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL8. After TMB substrate solution is added, only those wells that contain Human IL8 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL8 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include IL-8; CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1.