Cattle anti-HAV IgG(anti-Hepatitis A antibody IgG) ELISA Kit

Cattle anti-HAV IgG(anti-Hepatitis A antibody IgG) ELISA Kit is a research-use antibody from ELK Biotechnology for studying anti-HAV IgG in ELISA workflows with listed reactivity for Cattle. Specifications and supporting documents below should be reviewed when planning the experiment.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle Anti-HAV IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle Anti-HAV IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle Anti-HAV IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle Anti-HAV IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include Hepatitis A virus IgG.