14-3-3-pan (Acetyl Lys51/49) rabbit pAb from ELK Biotechnology Read more ›
14-3-3-pan (Acetyl Lys51/49) rabbit pAb is a research-use antibody from ELK Biotechnology for studying 14-3-3-pan in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008],
| Specificity | Acetyl-14-3-3-pan (K51/49) Polyclonal Antibody detects endogenous levels of 14-3-3-pan around the acetylation site of K51 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of MOUSE-SPLEEN using Acetyl-14-3-3-pan (K51/49) antibody. Antibody was diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
| Collaborator | ELK Biotechnology |
| Partner model | ES8431 |
| Target | 14-3-3-pan |
| Research areas | >>Cell cycle, >>Oocyte meiosis, >>PI3K-Akt signaling pathway, >>Hippo signaling pathway, >>Hepatitis C, >>Hepatitis B, >>Viral carcinogenesis |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized acetyl-peptide derived from human 14-3-3-pan around the acetylation site of K51. |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Colon carcinoma,Kerat |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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