A Cyclase VIII rabbit pAb from ELK Biotechnology Read more ›
A Cyclase VIII rabbit pAb is a research-use antibody from ELK Biotechnology for studying A Cyclase VIII in IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Adenylate cyclase is a membrane bound enzyme that catalyses the formation of cyclic AMP from ATP. The enzymatic activity is under the control of several hormones, and different polypeptides participate in the transduction of the signal from the receptor to the catalytic moiety. Stimulatory or inhibitory receptors (Rs and Ri) interact with G proteins (Gs and Gi) that exhibit GTPase activity and they modulate the activity of the catalytic subunit of the adenylyl cyclase [provided by RefSeq, Jul 2008],
| Specificity | A Cyclase VIII Polyclonal Antibody detects endogenous levels of A Cyclase VIII protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of A549 cells, using ADCY8 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human brain tissue, using ADCY8 Antibody. The picture on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4642 |
| Target | A Cyclase VIII |
| Research areas | >>Purine metabolism, >>Metabolic pathways, >>Endocrine resistance, >>Rap1 signaling pathway, >>Calcium signaling pathway, >>cGMP-PKG signaling pathway, >>cAMP signaling pathway, >>Chemokine signaling pathway, >>Phospholipase D signaling pathway, >>Oocyte meiosis, >>Longevity regulating pathway, >>Longevity regulating pathway - multiple species, >>Adrenergic signaling in cardiomyocytes, >>Vascular smooth muscle contraction, >>Apelin signaling pathway, >>Gap junction, >>Platelet activation, >>Circadian entrainment, >>Thermogenesis, >>Long-term potentiation, >>Retrograde endocannabinoid signaling, >>Glutamatergic synapse, >>Cholinergic synapse, >>GABAergic synapse, >>Taste transduction, >>Inflammatory mediator regulation of TRP channels, >>Insulin secretion, >>GnRH signaling pathway, >>Ovarian steroidogenesis, >>Progesterone-mediated oocyte maturation, >>Estrogen signaling pathway, >>Melanogenesis, >>Thyroid hormone synthesis, >>Oxytocin signaling pathway, >>Regulation of lipolysis in adipocytes, >>Aldosterone synthesis an |
| Reactivity | Human,Mouse,Rat |
| Applications | IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ADCY8. AA range:591-640 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Detected in brain cortex (PubMed:1715695). Expressed in islet (PubMed:25403481). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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