4E-BP1 (phospho Thr69) rabbit pAb from ELK Biotechnology Read more ›
4E-BP1 (phospho Thr69) rabbit pAb is a research-use antibody from ELK Biotechnology for studying 4E-BP1 in WB, IHC, IF, ELISA workflows with listed reactivity for Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
EIF4EBP1 encodes one member of a family of translation repressor proteins. The eukaryotic translation initiation factor 4E binding protein 1 directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
| Specificity | Phospho-4E-BP1 (T69) Polyclonal Antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at T69. |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using 4E-BP1 (Phospho-Thr69) Antibody | Immunohistochemistry analysis of paraffin-embedded human brain carcinoma, using 4E-BP1 (Phospho-Thr69) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using 4E-BP1 (Phospho-Thr69) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4385 |
| Target | 4E-BP1 |
| Research areas | Antibody Research |
| Reactivity | Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from mouse 4E-BP1 around the phosphorylation site of Thr69. AA range:35-84 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Mouse | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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