Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM95654

ABCF2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ABCF2 rabbit pAb from ELK Biotechnology Read more ›

ABCF2 rabbit pAb
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ABCF2 rabbit pAb

Product Description

ABCF2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ABCF2 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ATP-binding casette proteins transport various molecules across extra- and intracellular membranes. Alterations in this gene may be involved in cancer progression. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 7. [provided by RefSeq, Jul 2013],

Additional antibody information

Specificity ABCF2 Polyclonal Antibody detects endogenous levels of ABCF2 protein.
Validation evidence captions Western Blot analysis of various cells using ABCF2 Polyclonal Antibody | Western Blot analysis of HeLa cells using ABCF2 Polyclonal Antibody | Western blot analysis of ABCF2 Antibody. The lane on the right is blocked with the ABCF2 peptide. | Western blot analysis of the lysates from HepG2 cells using ABCF2 antibody.

Key Facts

Product typePrimary Antibody Target: ABCF2
Collaborator ELK Biotechnology
Partner model ES1568
Target ABCF2
Research areas>>Pathogenic Escherichia coli infection
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human ABCF2. AA range:171-220
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Colon,Lung,Uterus,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
ABCF2
Also known as
ABCF2; HUSSY-18; ATP-binding cassette sub-family F member 2; Iron-inhibited ABC transporter 2
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human ABCF2. AA range:171-220
Observed band
70kD
Cellular localization
nucleosome,nucleus,mitochondrion,mitochondrial envelope,membrane,ATP-binding cassette (ABC) transporter complex,
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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