Collaborator Elabscience
Primary AntibodiesISO 9001:2015ISO 14001:2015ISO 45001:2018Collaborator Tested
ABM-9984

Anti-Human CD45 Antibody [HI30]

Anti-Human CD45 Antibody [HI30] with collaborator-tested evidence for Flow Cyt, IHC. Read more ›

Human peripheral blood were stained with 0.2 μg Purified Anti-Human CD45 Antibody[HI30] (Right) and 0.2 μg Mouse IgG1, κ Isotype Control (Left), followed by FITC-conjugated Goat Anti-Mouse IgG Secondary Antibody.
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Human peripheral blood were stained with 0.2 μg Purified Anti-Human CD45 Antibody[HI30] (Right) and 0.2 μg Mouse IgG1, κ Isotype Control (Left), followed by FITC-conjugated Goat Anti-Mouse IgG Secondary Antibody.["FCM

Product Description

CD45 is the stated target for this antibody product. The listing uses collaborator-supplied technical data from Elabscience and is structured for research-use-only antibody selection through ABMIUM.

Product profile: reactivity: Human; host: Mouse; clonality: Monoclonal; clone: HI30; isotype: Mouse IgG1, κ; available conjugation options: Unconjugated, FITC, PE, APC, PerCP, PE/Cyanine 5, PE/Cyanine 7, PE/Cyanine 5.5, PerCP/Cyanine 5.5, Elab Fluor®488, Elab Fluor®647, Elab Fluor®700, PE/Elab Fluor®594, Elab Fluor®Violet 450, Elab Fluor®Violet 500, Elab Fluor®Red 780, Elab Fluor®Violet 540.

Application evidence: Elabscience lists this product for FCM, IHC, FCM. In the ABMIUM matrix, these listed applications are marked C to show collaborator-tested ABMIUM Verified evidence. Applications not listed in the supplied data remain marked N.

Technical notes: immunogen: Recombinant Human CD45 protein; purification: >98%, Protein A/G purified; concentration: ≥ 1 mg/mL.

Database references: UniProt: P08575 | NCBI Gene: 5788.

Research Use Only.

Key Facts

Product typePrimary Antibodies Target: CD45
Collaborator Elabscience
Partner model E-AB-F1137A_100mg
Target CD45
Research areasCell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Reactivity Human
Applications Flow Cytometry,IHC
ConjugationUnconjugated, FITC, PE, APC, PerCP, PE/Cyanine 5, PE/Cyanine 7, PE/Cyanine 5.5, PerCP/Cyanine 5.5, Elab Fluor®488, Elab Fluor®647, Elab Fluor®700, PE/Elab Fluor®594, Elab Fluor®Violet 450, Elab Fluor®Violet 500, Elab Fluor®Red 780, Elab Fluor®Violet 540
Dilution rangeFCM 2 μg/mL(0.5×10<sup>6</sup>-1×10<sup>6</sup> cells), IHC 1:50-1:100
Host Mouse
Clonality Monoclonal
Clone HI30
Isotype Mouse IgG1, κ
Immunogen Recombinant Human CD45 protein
Concentration ≥ 1 mg/mL
Purification >98%, Protein A/G purified
Purity >98%
Shelf life 12 months from shipping dates
AvailabilityIn stock - ships within 2 business days
Lead time2-3 business days
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHC-PIHC-FrIF/ICCFlow CytIPChIPIHC
Human

Specifications

Target
CD45
Also known as
CD45, L-CA, Ly-5, Ptprc, Receptor-type tyrosine-protein phosphatase C, T200
Concentration
≥ 1 mg/mL
Purity
>98%
Host species
Mouse
Clonality
Monoclonal
Clone number
HI30
Isotype
Mouse IgG1, κ
Conjugation
Unconjugated, FITC, PE, APC, PerCP, PE/Cyanine 5, PE/Cyanine 7, PE/Cyanine 5.5, PerCP/Cyanine 5.5, Elab Fluor®488, Elab Fluor®647, Elab Fluor®700, PE/Elab Fluor®594, Elab Fluor®Violet 450, Elab Fluor®Violet 500, Elab Fluor®Red 780, Elab Fluor®Violet 540
Species reactivity
Human
Tested applications
Flow CytometryIHC
Immunogen
Recombinant Human CD45 protein
Dilution range
FCM 2 μg/mL(0.5×106-1×106 cells), IHC 1:50-1:100
Purification
>98%, Protein A/G purified

Storage & Stability

Shipped at
Wet ice (2-8 degrees C)
Short-term storage
2-8 degrees C for up to 1 month
Long-term storage
4 degrees C for up to 12 months. Do not freeze.
Storage buffer
Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling.
Aliquoting advice
Do not freeze. Aliquot only if necessary to avoid repeated opening.
Shelf life
12 months from shipping dates

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001:2015

Quality management certification.

ISO
ISO 14001:2015

Environmental management certification.

ISO
ISO 45001:2018

Occupational health and safety management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

REACH
REACH

REACH compliant.

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