Collaborator Elabscience
Primary AntibodiesISO 9001:2015ISO 14001:2015ISO 45001:2018Collaborator Tested
ABM-9974

Anti-Human/Monkey CD154 Antibody [5C8]

Anti-Human/Monkey CD154 Antibody [5C8] with collaborator-tested evidence for Flow Cyt. Read more ›

HEK293T cells transfected with pcDNA3.1 plasmid encoding Human CD154 gene were stained with 0.2 μg AF/LE Purified Anti-Human CD154 Antibody[5C8] (Right) and 0.2 μg Mouse IgG2a, κ Isotype Control (Left), followed by APC-conjugated Goat Anti-Mouse IgG Secondary Antibody.
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HEK293T cells transfected with pcDNA3.1 plasmid encoding Human CD154 gene were stained with 0.2 μg AF/LE Purified Anti-Human CD154 Antibody[5C8] (Right) and 0.2 μg Mouse IgG2a, κ Isotype Control (Left), followed by APC-conjugated Goat Anti-Mouse IgG Secondary Antibody.["FCM"]

Product Description

CD154 is the stated target for this antibody product. The listing uses collaborator-supplied technical data from Elabscience and is structured for research-use-only antibody selection through ABMIUM.

Product profile: reactivity: Human, Monkey; host: Mouse; clonality: Monoclonal; clone: 5C8; isotype: Mouse IgG2a, κ; available conjugation options: None (AF/LE), Unconjugated.

Application evidence: Elabscience lists this product for FCM. In the ABMIUM matrix, these listed applications are marked C to show collaborator-tested ABMIUM Verified evidence. Applications not listed in the supplied data remain marked N.

Technical notes: immunogen: Recombinant Human CD154 protein; purification: >98%, Protein A/G purified; concentration: ≥ 1 mg/mL.

Database references: UniProt: P29965 | NCBI Gene: 959.

Research Use Only.

Key Facts

Product typePrimary Antibodies Target: CD154
Collaborator Elabscience
Partner model E-AB-F11280_100mg
Target CD154
Research areasCostimulatory Molecules, Immunology
Reactivity Human,Monkey
Applications Flow Cytometry
ConjugationNone (AF/LE), Unconjugated
Dilution rangeFCM 2 μg/mL(0.5×10<sup>6</sup>-1×10<sup>6</sup> cells)
Host Mouse
Clonality Monoclonal
Clone 5C8
Isotype Mouse IgG2a, κ
Immunogen Recombinant Human CD154 protein
Concentration ≥ 1 mg/mL
Purification >98%, Protein A/G purified
Purity >98%
Shelf life 12 months from shipping dates
AvailabilityIn stock - ships within 2 business days
Lead time2-3 business days
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHC-PIHC-FrIF/ICCFlow CytIPChIP
Human
Monkey

Specifications

Target
CD154
Also known as
T-BAM, TNFSF5, TRAP, gp39, CD40L
Concentration
≥ 1 mg/mL
Purity
>98%
Host species
Mouse
Clonality
Monoclonal
Clone number
5C8
Isotype
Mouse IgG2a, κ
Conjugation
None (AF/LE), Unconjugated
Species reactivity
Human,Monkey
Tested applications
Flow Cytometry
Immunogen
Recombinant Human CD154 protein
Dilution range
FCM 2 μg/mL(0.5×106-1×106 cells)
Purification
>98%, Protein A/G purified

Storage & Stability

Shipped at
Wet ice (2-8 degrees C)
Short-term storage
2-8 degrees C for up to 1 month
Long-term storage
4 degrees C for up to 12 months. Do not freeze.
Storage buffer
Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.
Aliquoting advice
Do not freeze. Aliquot only if necessary to avoid repeated opening.
Shelf life
12 months from shipping dates

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001:2015

Quality management certification.

ISO
ISO 14001:2015

Environmental management certification.

ISO
ISO 45001:2018

Occupational health and safety management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

REACH
REACH

REACH compliant.

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