Collaborator Elabscience
Primary AntibodiesISO 9001:2015ISO 14001:2015ISO 45001:2018Collaborator Tested
ABM-9947

Anti-Mouse CD8a Antibody [53-6.7]

Anti-Mouse CD8a Antibody [53-6.7] with collaborator-tested evidence for Flow Cyt. Read more ›

C57/BL6 Mouse splenocytes were stained with 0.2 μg AF/LE Purified Anti-Mouse CD8a Antibody[53-6.7] (Right) and 0.2 μg Rat IgG2a, κ Isotype Control (Left), followed by Elab Fluor® 647-conjugated Goat Anti-Rat IgG Secondary Antibody, then anti-Mouse CD4 FITC-conjugated Monoclonal Antibody.
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C57/BL6 Mouse splenocytes were stained with 0.2 μg AF/LE Purified Anti-Mouse CD8a Antibody[53-6.7] (Right) and 0.2 μg Rat IgG2a, κ Isotype Control (Left), followed by Elab Fluor® 647-conjugated Goat Anti-Rat IgG Secondary Antibody, then anti-Mouse CD4 FITC-conjugated Monoclonal Antibody.["FCM"]

Product Description

CD8a is the stated target for this antibody product. The listing uses collaborator-supplied technical data from Elabscience and is structured for research-use-only antibody selection through ABMIUM.

Product profile: reactivity: Mouse; host: Rat; clonality: Monoclonal; clone: 53-6.7; isotype: Rat IgG2a, κ; available conjugation options: None (AF/LE), Unconjugated, Biotin, FITC, PE, APC, PerCP, PE/Cyanine 7, PE/Cyanine 5.5, PerCP/Cyanine 5.5, Elab Fluor®488, Elab Fluor®647, Elab Fluor®700, PE/Elab Fluor®594, Elab Fluor®Violet 450, Elab Bright™Violet 421, Elab Fluor®Violet 500, Elab Bright™Violet 510, Elab Fluor®Red 780, Elab Fluor®Violet 540, Elab Bright™Violet 650.

Application evidence: Elabscience lists this product for FCM. In the ABMIUM matrix, these listed applications are marked C to show collaborator-tested ABMIUM Verified evidence. Applications not listed in the supplied data remain marked N.

Technical notes: immunogen: Recombinant Mouse CD8a protein; purification: >98%, Protein A/G purified; concentration: ≥ 1 mg/mL.

Database references: UniProt: P01731 | NCBI Gene: 12525.

Research Use Only.

Key Facts

Product typePrimary Antibodies Target: CD8a
Collaborator Elabscience
Partner model E-AB-F11040_100mg
Target CD8a
Reactivity Mouse
Applications Flow Cytometry
ConjugationNone (AF/LE), Unconjugated, Biotin, FITC, PE, APC, PerCP, PE/Cyanine 7, PE/Cyanine 5.5, PerCP/Cyanine 5.5, Elab Fluor®488, Elab Fluor®647, Elab Fluor®700, PE/Elab Fluor®594, Elab Fluor®Violet 450, Elab Bright™Violet 421, Elab Fluor®Violet 500, Elab Bright™Violet 510, Elab Fluor®Red 780, Elab Fluor®Violet 540, Elab Bright™Violet 650
Dilution rangeFCM 2 μg/mL(0.5×10<sup>6</sup>-1×10<sup>6</sup> cells)
Host Rat
Clonality Monoclonal
Clone 53-6.7
Isotype Rat IgG2a, κ
Immunogen Recombinant Mouse CD8a protein
Concentration ≥ 1 mg/mL
Purification >98%, Protein A/G purified
Purity >98%
Shelf life 12 months from shipping dates
AvailabilityIn stock - ships within 2 business days
Lead time2-3 business days
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHC-PIHC-FrIF/ICCFlow CytIPChIP
Mouse

Specifications

Target
CD8a
Also known as
CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Concentration
≥ 1 mg/mL
Purity
>98%
Host species
Rat
Clonality
Monoclonal
Clone number
53-6.7
Isotype
Rat IgG2a, κ
Conjugation
None (AF/LE), Unconjugated, Biotin, FITC, PE, APC, PerCP, PE/Cyanine 7, PE/Cyanine 5.5, PerCP/Cyanine 5.5, Elab Fluor®488, Elab Fluor®647, Elab Fluor®700, PE/Elab Fluor®594, Elab Fluor®Violet 450, Elab Bright™Violet 421, Elab Fluor®Violet 500, Elab Bright™Violet 510, Elab Fluor®Red 780, Elab Fluor®Violet 540, Elab Bright™Violet 650
Species reactivity
Mouse
Tested applications
Flow Cytometry
Immunogen
Recombinant Mouse CD8a protein
Dilution range
FCM 2 μg/mL(0.5×106-1×106 cells)
Purification
>98%, Protein A/G purified

Storage & Stability

Shipped at
Wet ice (2-8 degrees C)
Short-term storage
2-8 degrees C for up to 1 month
Long-term storage
4 degrees C for up to 12 months. Do not freeze.
Storage buffer
Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.
Aliquoting advice
Do not freeze. Aliquot only if necessary to avoid repeated opening.
Shelf life
12 months from shipping dates

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001:2015

Quality management certification.

ISO
ISO 14001:2015

Environmental management certification.

ISO
ISO 45001:2018

Occupational health and safety management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

REACH
REACH

REACH compliant.

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