Collaborator Elabscience
Primary AntibodiesISO 9001:2015ISO 14001:2015ISO 45001:2018Collaborator Tested
ABM-9865

Anti-Mouse CD16/32 Antibody [2.4G2]

Anti-Mouse CD16/32 Antibody [2.4G2] with collaborator-tested evidence for Flow Cyt, Block. Read more ›

C57/BL6 Mouse splenocytes were stained with 0.2 μg AF/LE Purified Anti-Mouse CD16/32 Antibody[2.4G2] (Right) and 0.2 μg Rat IgG2b, κ Isotype Control (Left), followed by Elab Fluor® 647-conjugated Goat Anti- Rat IgG Secondary Antibody, then anti-Mouse B220 PE-conjugated Monoclonal Antibody.
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C57/BL6 Mouse splenocytes were stained with 0.2 μg AF/LE Purified Anti-Mouse CD16/32 Antibody[2.4G2] (Right) and 0.2 μg Rat IgG2b, κ Isotype Control (Left), followed by Elab Fluor® 647-conjugated Goat Anti- Rat IgG Secondary Antibody, then anti-Mouse B220 PE-conjugated Monoclonal Antibody.["FCM"]

Product Description

CD16/32 is the stated target for this antibody product. The listing uses collaborator-supplied technical data from Elabscience and is structured for research-use-only antibody selection through ABMIUM.

Product profile: reactivity: Mouse; host: Rat; clonality: Monoclonal; clone: 2.4G2; isotype: Rat IgG2b, κ; available conjugation options: None (AF/LE), Unconjugated, Biotin, FITC, PE, APC, PE/Cyanine 5, PE/Cyanine 7, PE/Cyanine 5.5, PerCP/Cyanine 5.5, Elab Fluor®488, Elab Fluor®647, Elab Fluor®700, PE/Elab Fluor®594, Elab Fluor®Violet 450, Elab Fluor®Violet 500, Elab Fluor®Red 780, Elab Fluor®Violet 540.

Application evidence: Elabscience lists this product for FCM, FCM, Block. In the ABMIUM matrix, these listed applications are marked C to show collaborator-tested ABMIUM Verified evidence. Applications not listed in the supplied data remain marked N.

Technical notes: immunogen: Recombinant Mouse CD16/32 protein; purification: >98%, Protein A/G purified; concentration: ≥ 1 mg/mL.

Database references: UniProt: P08508;P08101; | NCBI Gene: 14130.

Research Use Only.

Key Facts

Product typePrimary Antibodies Target: CD16/32
Collaborator Elabscience
Partner model E-AB-F09970_100mg
Target CD16/32
Research areasImmunology, Innate Immunity
Reactivity Mouse
Applications Flow Cytometry,Block
ConjugationNone (AF/LE), Unconjugated, Biotin, FITC, PE, APC, PE/Cyanine 5, PE/Cyanine 7, PE/Cyanine 5.5, PerCP/Cyanine 5.5, Elab Fluor®488, Elab Fluor®647, Elab Fluor®700, PE/Elab Fluor®594, Elab Fluor®Violet 450, Elab Fluor®Violet 500, Elab Fluor®Red 780, Elab Fluor®Violet 540
Dilution rangeFCM 2 μg/mL(0.5×10<sup>6</sup>-1×10<sup>6</sup> cells), Block 0.5-1 μg(0.5×10<sup>6</sup>-1×10<sup>6</sup> cells) in 100 μL
Host Rat
Clonality Monoclonal
Clone 2.4G2
Isotype Rat IgG2b, κ
Immunogen Recombinant Mouse CD16/32 protein
Concentration ≥ 1 mg/mL
Purification >98%, Protein A/G purified
Purity >98%
Shelf life 12 months from shipping dates
AvailabilityIn stock - ships within 2 business days
Lead time2-3 business days
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHC-PIHC-FrIF/ICCFlow CytIPChIPBlock
Mouse

Specifications

Target
CD16/32
Also known as
CD16a/b, CD32, CD32A/B, FCG2A, FCGR2A/BFCGR3, FCGR3A/B, Fc fragment of IgG low affinity IIIa/b receptor, Fc fragment of IgG low affinity IIIb receptor, Fc fragment of IgG low affinity IIa/b receptor, Fc gamma RIIa/b , Fc gamma receptor III A/B, FcGR
Concentration
≥ 1 mg/mL
Purity
>98%
Host species
Rat
Clonality
Monoclonal
Clone number
2.4G2
Isotype
Rat IgG2b, κ
Conjugation
None (AF/LE), Unconjugated, Biotin, FITC, PE, APC, PE/Cyanine 5, PE/Cyanine 7, PE/Cyanine 5.5, PerCP/Cyanine 5.5, Elab Fluor®488, Elab Fluor®647, Elab Fluor®700, PE/Elab Fluor®594, Elab Fluor®Violet 450, Elab Fluor®Violet 500, Elab Fluor®Red 780, Elab Fluor®Violet 540
Species reactivity
Mouse
Tested applications
Flow CytometryBlock
Immunogen
Recombinant Mouse CD16/32 protein
Dilution range
FCM 2 μg/mL(0.5×106-1×106 cells), Block 0.5-1 μg(0.5×106-1×106 cells) in 100 μL
Purification
>98%, Protein A/G purified

Storage & Stability

Shipped at
Wet ice (2-8 degrees C)
Short-term storage
2-8 degrees C for up to 1 month
Long-term storage
4 degrees C for up to 12 months. Do not freeze.
Storage buffer
Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.
Aliquoting advice
Do not freeze. Aliquot only if necessary to avoid repeated opening.
Shelf life
12 months from shipping dates

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001:2015

Quality management certification.

ISO
ISO 14001:2015

Environmental management certification.

ISO
ISO 45001:2018

Occupational health and safety management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

REACH
REACH

REACH compliant.

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