Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM93715

ABCA1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ABCA1 rabbit pAb from ELK Biotechnology Read more ›

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Product Description

ABCA1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ABCA1 in WB, ELISA workflows with listed reactivity for Human, Mouse, Golden hamster. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity ABCA1 Polyclonal Antibody detects endogenous levels of protein.

Key Facts

Product typePrimary Antibody Target: ABCA1
Collaborator ELK Biotechnology
Partner model ES11793
Target ABCA1
Research areas>>ABC transporters, >>Fat digestion and absorption, >>Cholesterol metabolism, >>Lipid and atherosclerosis
Reactivity Human,Mouse,Golden hamster
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000 ELISA 1:5000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from part region of human protein AA range: 1112-1180
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Widely expressed, but most abundant in macrophages.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Golden hamster
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 ELISA 1:5000-20000
Mouse
Golden hamster
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
ABCA1
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Golden hamster
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from part region of human protein AA range: 1112-1180
Observed band
248kD
Cellular localization
Cell membrane ; Multi-pass membrane protein . Endosome .
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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