Collaborator Elabscience
Primary AntibodiesRecombinantISO 9001:2015ISO 14001:2015ISO 45001:2018Collaborator Tested
ABM-9330

Anti-Human Semaphorin 4D Antibody [R05-2E3]

Anti-Human Semaphorin 4D Antibody [R05-2E3] with collaborator-tested evidence for WB. Read more ›

Western blot detection of Semaphorin 4D/CD100 in Jurkat cell lysates using Semaphorin 4D/CD100 Rabbit mAb(1:500 diluted).Predicted band size:96kDa.Observed band size:120kDa.
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Western blot detection of Semaphorin 4D/CD100 in Jurkat cell lysates using Semaphorin 4D/CD100 Rabbit mAb(1:500 diluted).Predicted band size:96kDa.Observed band size:120kDa.["WB"]

Product Description

Semaphorin 4D is the stated target for this antibody product. The listing uses collaborator-supplied technical data from Elabscience and is structured for research-use-only antibody selection through ABMIUM.

Product profile: reactivity: Human; host: Rabbit; clonality: Monoclonal; clone: R05-2E3; isotype: IgG; available conjugation options: Unconjugated.

Application evidence: Elabscience lists this product for WB. In the ABMIUM matrix, these listed applications are marked C to show collaborator-tested ABMIUM Verified evidence. Applications not listed in the supplied data remain marked N.

Technical notes: immunogen: A synthetic peptide of human Semaphorin 4D; purification: Affinity Purified; concentration: 300 μg/mL.

Database references: UniProt: Q92854 | NCBI Gene: 10507.

Research Use Only.

Key Facts

Product typePrimary Antibodies Target: Semaphorin 4D
Collaborator Elabscience
Partner model E-AB-81485_100μL
Target Semaphorin 4D
Research areasCancer, Immunology, Neuroscience
Reactivity Human
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:1000-1:5000
Host Rabbit
Clonality Monoclonal
Clone R05-2E3
Isotype IgG
Immunogen A synthetic peptide of human Semaphorin 4D
Concentration 300 μg/mL
Purification Affinity Purified
Shelf life 12 months from shipping dates
AvailabilityIn stock - ships within 2 business days
Lead time2-3 business days
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHC-PIHC-FrIF/ICCFlow CytIPChIP
Human

Specifications

Target
Semaphorin 4D
Also known as
A8, BB 18, BB18, CD 100, CD100, CD100 antigen, COLL4, Coll 4, Collapsin 4, Collapsin4, GR3, Leukocyte activation antigen CD100, M sema G, MSEMA, SEM4D, Sema 4d, Sema H, Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4D
Concentration
300 μg/mL
Host species
Rabbit
Clonality
Monoclonal
Clone number
R05-2E3
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human
Tested applications
WB
Immunogen
A synthetic peptide of human Semaphorin 4D
Observed band
120 kDa
Dilution range
WB 1:1000-1:5000
Purification
Affinity Purified

Storage & Stability

Shipped at
Wet ice (2-8 degrees C)
Short-term storage
2-8 degrees C for up to 1 month
Long-term storage
4 degrees C for up to 12 months. Do not freeze.
Storage buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein.
Aliquoting advice
Do not freeze. Aliquot only if necessary to avoid repeated opening.
Shelf life
12 months from shipping dates

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001:2015

Quality management certification.

ISO
ISO 14001:2015

Environmental management certification.

ISO
ISO 45001:2018

Occupational health and safety management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

REACH
REACH

REACH compliant.

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