Collaborator Elabscience
Primary AntibodiesRecombinantISO 9001:2015ISO 14001:2015ISO 45001:2018Collaborator Tested
ABM-9312

Anti-Human Estrogen Receptor alpha Antibody [R05-1E4]

Anti-Human Estrogen Receptor alpha Antibody [R05-1E4] with collaborator-tested evidence for WB, IHC-P, IF/ICC. Read more ›

Western blot detection of Estrogen Receptor alpha in K562,Hela cell lysates using Estrogen Receptor alpha Rabbit mAb(1:1000 diluted).Predicted band size:66kDa.Observed band size:66kDa.
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Western blot detection of Estrogen Receptor alpha in K562,Hela cell lysates using Estrogen Receptor alpha Rabbit mAb(1:1000 diluted).Predicted band size:66kDa.Observed band size:66kDa.["WB

Product Description

Estrogen Receptor alpha is the stated target for this antibody product. The listing uses collaborator-supplied technical data from Elabscience and is structured for research-use-only antibody selection through ABMIUM.

Product profile: reactivity: Human; host: Rabbit; clonality: Monoclonal; clone: R05-1E4; isotype: IgG; available conjugation options: Unconjugated.

Application evidence: Elabscience lists this product for WB, IHC-P, IF. In the ABMIUM matrix, these listed applications are marked C to show collaborator-tested ABMIUM Verified evidence. Applications not listed in the supplied data remain marked N.

Technical notes: immunogen: A synthetic peptide of human Estrogen Receptor alpha; purification: Affinity Purified; concentration: 300 μg/mL.

Database references: UniProt: P03372 | NCBI Gene: 2099.

Research Use Only.

Key Facts

Product typePrimary Antibodies Target: Estrogen Receptor alpha
Collaborator Elabscience
Partner model E-AB-81467_100μL
Target Estrogen Receptor alpha
Research areasCancer, Epigenetics and Nuclear Signaling, Neuroscience, Signal Transduction
Reactivity Human
Applications WB,IHC-P,IF/ICC
ConjugationUnconjugated
Dilution rangeWB 1:500-1:1000, IHC 1:50-1:100, IF 1:20-1:100
Host Rabbit
Clonality Monoclonal
Clone R05-1E4
Isotype IgG
Immunogen A synthetic peptide of human Estrogen Receptor alpha
Concentration 300 μg/mL
Purification Affinity Purified
Shelf life 12 months from shipping dates
AvailabilityIn stock - ships within 2 business days
Lead time2-3 business days
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHC-PIHC-FrIF/ICCFlow CytIPChIP
Human

Specifications

Target
Estrogen Receptor alpha
Also known as
4, 5, 6, 7*, 7*/822 isoform, 8*/941 isof, DKFZp686N23123, ER, ER alpha, ER-alpha, ESR, ESR1, ESRA, Era, Estradiol receptor, Estrogen nuclear receptor alpha, Estrogen receptor 1, Estrogen receptor alpha, Estrogen receptor alpha 3*, Estrogen receptor alpha delta 3*
Concentration
300 μg/mL
Host species
Rabbit
Clonality
Monoclonal
Clone number
R05-1E4
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human
Tested applications
WBIHC-PIF/ICC
Immunogen
A synthetic peptide of human Estrogen Receptor alpha
Observed band
66 kDa
Dilution range
WB 1:500-1:1000, IHC 1:50-1:100, IF 1:20-1:100
Purification
Affinity Purified

Storage & Stability

Shipped at
Wet ice (2-8 degrees C)
Short-term storage
2-8 degrees C for up to 1 month
Long-term storage
4 degrees C for up to 12 months. Do not freeze.
Storage buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein.
Aliquoting advice
Do not freeze. Aliquot only if necessary to avoid repeated opening.
Shelf life
12 months from shipping dates

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001:2015

Quality management certification.

ISO
ISO 14001:2015

Environmental management certification.

ISO
ISO 45001:2018

Occupational health and safety management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

REACH
REACH

REACH compliant.

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