Collaborator Elabscience
Primary AntibodiesISO 9001:2015ISO 14001:2015ISO 45001:2018Collaborator Tested
ABM-7575

Anti-Human/Mouse/Rat ERK 1/2 Antibody

Anti-Human/Mouse/Rat ERK 1/2 Antibody with collaborator-tested evidence for WB, IF/ICC. Read more ›

Western Blot analysis of HepG2, Hela cells using ERK 1/2 Polyclonal Antibody at dilution of 1:1000.
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Western Blot analysis of HepG2, Hela cells using ERK 1/2 Polyclonal Antibody at dilution of 1:1000.["WB

Product Description

ERK 1/2 is the stated target for this antibody product. The listing uses collaborator-supplied technical data from Elabscience and is structured for research-use-only antibody selection through ABMIUM.

Product profile: reactivity: Human, Mouse, Rat; host: Rabbit; clonality: Polyclonal; isotype: IgG; available conjugation options: Unconjugated.

Application evidence: Elabscience lists this product for WB, IF. In the ABMIUM matrix, these listed applications are marked C to show collaborator-tested ABMIUM Verified evidence. Applications not listed in the supplied data remain marked N.

Technical notes: immunogen: Synthesized peptide derived from human ERK 1/2 around the non-phosphorylation site of Thr202.; purification: Affinity purification; concentration: 1 mg/mL.

Database references: UniProt: P27361;P28482 | NCBI Gene: 5594;5595.

Research Use Only.

Key Facts

Product typePrimary Antibodies Target: ERK 1/2
Collaborator Elabscience
Partner model E-AB-31372_120μL
Target ERK 1/2
Research areasCancer, Signal Transduction, Neuroscience
Reactivity Human,Mouse,Rat
Applications WB,IF/ICC
ConjugationUnconjugated
Dilution rangeWB 1:500-1:2000, IF 1:100-1:300
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human ERK 1/2 around the non-phosphorylation site of Thr202.
Concentration 1 mg/mL
Purification Affinity purification
Shelf life 12 months from shipping dates
AvailabilityIn stock - ships within 2 business days
Lead time2-3 business days
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHC-PIHC-FrIF/ICCFlow CytIPChIP
Human
Mouse
Rat

Specifications

Target
ERK 1/2
Also known as
ERK-1, ERK1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, MAPK3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 3, PRKM3, p, p44-MAPK
Concentration
1 mg/mL
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBIF/ICC
Immunogen
Synthesized peptide derived from human ERK 1/2 around the non-phosphorylation site of Thr202.
Observed band
44+42 kDa
Dilution range
WB 1:500-1:2000, IF 1:100-1:300
Purification
Affinity purification

Storage & Stability

Shipped at
Wet ice (2-8 degrees C)
Short-term storage
2-8 degrees C for up to 1 month
Long-term storage
4 degrees C for up to 12 months. Do not freeze.
Storage buffer
Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol.
Aliquoting advice
Do not freeze. Aliquot only if necessary to avoid repeated opening.
Shelf life
12 months from shipping dates

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001:2015

Quality management certification.

ISO
ISO 14001:2015

Environmental management certification.

ISO
ISO 45001:2018

Occupational health and safety management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

REACH
REACH

REACH compliant.

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