Collaborator Elabscience
Primary AntibodiesISO 9001:2015ISO 14001:2015ISO 45001:2018Collaborator Tested
ABM-7475

Anti-Human HSP27 Antibody [3E5]

Anti-Human HSP27 Antibody [3E5] with collaborator-tested evidence for WB, IHC-P. Read more ›

Western Blot analysis of Hela cells using HSP27 Monoclonal Antibody at dilution of 1:2000.
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Western Blot analysis of Hela cells using HSP27 Monoclonal Antibody at dilution of 1:2000.["WB

Product Description

HSP27 is the stated target for this antibody product. The listing uses collaborator-supplied technical data from Elabscience and is structured for research-use-only antibody selection through ABMIUM.

Product profile: reactivity: Human; host: Mouse; clonality: Monoclonal; clone: 3E5; isotype: IgG; available conjugation options: Unconjugated.

Application evidence: Elabscience lists this product for WB, IHC-p. In the ABMIUM matrix, these listed applications are marked C to show collaborator-tested ABMIUM Verified evidence. Applications not listed in the supplied data remain marked N.

Technical notes: immunogen: Recombinant Protein; purification: Protein A purification; concentration: 1 mg/mL.

Database references: UniProt: P04792 | NCBI Gene: 3315.

Research Use Only.

Key Facts

Product typePrimary Antibodies Target: HSP27
Collaborator Elabscience
Partner model E-AB-22157_120μL
Target HSP27
Research areasCancer, Cardiovascular, Signal Transduction
Reactivity Human
Applications WB,IHC-P
ConjugationUnconjugated
Dilution rangeWB 1:1000-1:3000, IHC 1:50-1:200
Host Mouse
Clonality Monoclonal
Clone 3E5
Isotype IgG
Immunogen Recombinant Protein
Concentration 1 mg/mL
Purification Protein A purification
Shelf life 12 months from shipping dates
AvailabilityIn stock - ships within 2 business days
Lead time2-3 business days
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHC-PIHC-FrIF/ICCFlow CytIPChIP
Human

Specifications

Target
HSP27
Also known as
28 kDa heat shock protein, CMT2F, DKFZp586P1322, Estrogen regulated 24 kDa protein, Estrogen-regulated 24 kDa protein, H, Heat shock 25kDa protein 1, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27kDa protein, epididymis secretory protein Li 102
Concentration
1 mg/mL
Host species
Mouse
Clonality
Monoclonal
Clone number
3E5
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human
Tested applications
WBIHC-P
Immunogen
Recombinant Protein
Observed band
27 kDa
Dilution range
WB 1:1000-1:3000, IHC 1:50-1:200
Purification
Protein A purification

Storage & Stability

Shipped at
Wet ice (2-8 degrees C)
Short-term storage
2-8 degrees C for up to 1 month
Long-term storage
4 degrees C for up to 12 months. Do not freeze.
Storage buffer
Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol.
Aliquoting advice
Do not freeze. Aliquot only if necessary to avoid repeated opening.
Shelf life
12 months from shipping dates

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001:2015

Quality management certification.

ISO
ISO 14001:2015

Environmental management certification.

ISO
ISO 45001:2018

Occupational health and safety management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

REACH
REACH

REACH compliant.

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