Collaborator Elabscience
Primary AntibodiesISO 9001:2015ISO 14001:2015ISO 45001:2018Collaborator Tested
ABM-7440

Anti-Human/Mouse/Rat Active Caspase-3 Antibody [5A2]

Anti-Human/Mouse/Rat Active Caspase-3 Antibody [5A2] with collaborator-tested evidence for WB, IHC-P, IF/ICC. Read more ›

Western Blot analysis of 1) Hela, 2) 3T3, 3) Rat brain with Active CASP3 Monoclonal Antibody
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Western Blot analysis of 1) Hela, 2) 3T3, 3) Rat brain with Active CASP3 Monoclonal Antibody["WB

Product Description

Active Caspase-3 is the stated target for this antibody product. The listing uses collaborator-supplied technical data from Elabscience and is structured for research-use-only antibody selection through ABMIUM.

Product profile: reactivity: Human, Mouse, Rat; host: Mouse; clonality: Monoclonal; clone: 5A2; isotype: IgG; available conjugation options: Unconjugated.

Application evidence: Elabscience lists this product for WB, IHC-p, IF. In the ABMIUM matrix, these listed applications are marked C to show collaborator-tested ABMIUM Verified evidence. Applications not listed in the supplied data remain marked N.

Technical notes: immunogen: Recombinant Protein; purification: Protein A purification; concentration: 1 mg/mL.

Database references: UniProt: P42574 | NCBI Gene: 836.

Research Use Only.

Key Facts

Product typePrimary Antibodies Target: Active Caspase-3
Collaborator Elabscience
Partner model E-AB-22115_120μL
Target Active Caspase-3
Research areasCancer, Cell Biology, Metabolism, Neuroscience
Reactivity Human,Mouse,Rat
Applications WB,IHC-P,IF/ICC
ConjugationUnconjugated
Dilution rangeWB 1:500-1:1000, IHC 1:100-1:200, IF 1:100-1:200
Host Mouse
Clonality Monoclonal
Clone 5A2
Isotype IgG
Immunogen Recombinant Protein
Concentration 1 mg/mL
Purification Protein A purification
Shelf life 12 months from shipping dates
AvailabilityIn stock - ships within 2 business days
Lead time2-3 business days
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHC-PIHC-FrIF/ICCFlow CytIPChIP
Human
Mouse
Rat

Specifications

Target
Active Caspase-3
Also known as
A830040C14Rik, Apopain, CASP-3, CASP3, CC3, CPP-32, CPP32, CPP32B, Casp3a, Caspase 3, Caspase-3 subunit p12, Cysteine p, apoptosis-related cysteine peptidase, apoptosis-related cysteine protease, apoptosis-related cysteine protease a
Concentration
1 mg/mL
Host species
Mouse
Clonality
Monoclonal
Clone number
5A2
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBIHC-PIF/ICC
Immunogen
Recombinant Protein
Observed band
17 kDa
Dilution range
WB 1:500-1:1000, IHC 1:100-1:200, IF 1:100-1:200
Purification
Protein A purification

Storage & Stability

Shipped at
Wet ice (2-8 degrees C)
Short-term storage
2-8 degrees C for up to 1 month
Long-term storage
4 degrees C for up to 12 months. Do not freeze.
Storage buffer
Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol.
Aliquoting advice
Do not freeze. Aliquot only if necessary to avoid repeated opening.
Shelf life
12 months from shipping dates

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001:2015

Quality management certification.

ISO
ISO 14001:2015

Environmental management certification.

ISO
ISO 45001:2018

Occupational health and safety management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

REACH
REACH

REACH compliant.

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