EasyStep Rat 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit from ELK Biotechnology Read more ›
EasyStep Rat 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring EasyStep Rat 25-OH-D in Serum, Plasma samples from Rat. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 25-OH-D antigen, and the Rat 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative names include 25 Hydroxy Vitamin D;.
| Collaborator | ELK Biotechnology |
| Partner model | ELK053ES |
| Target | EasyStep Rat 25-OH-D |
| Research areas | Reproductive science, Genetic science, Nutrition metabolism, Bone metabolism |
| Reactivity | Rat |
| Applications | ELISA |
| Assay type | Competitive Inhibition |
| Sample types | Serum,Plasma |
| Detection range | 78.13-5000 ng/mL |
| Sensitivity | 7.7 ng/mL |
| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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