EasyStep Rat fT3(Free Triiodothyronine) ELISA Kit from ELK Biotechnology Read more ›
EasyStep Rat fT3(Free Triiodothyronine) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring EasyStep Rat fT3 in Serum, Plasma samples from Rat. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat fT3 antigen, and the Rat fT3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat fT3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat fT3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative names include 3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin.
| Collaborator | ELK Biotechnology |
| Partner model | ELK050ES |
| Target | EasyStep Rat fT3 |
| Research areas | Endocrinology, Autoimmunity |
| Reactivity | Rat |
| Applications | ELISA |
| Assay type | Competitive Inhibition |
| Sample types | Serum,Plasma |
| Detection range | 0.32-20 ng/mL |
| Sensitivity | 0.31 ng/mL |
| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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