Home » Recombinant SARS-CoV-2 Spike S1+S2 ECD (ΔHV69-70,ΔY144,N501Y,A570D,D614G,P681H,T716I,S982A,D1118H)(His Tag)
Recombinant Proteins ISO 9001:2015ISO 14001:2015ISO 45001:2018ABMIUM Verified™Collaborator Tested
ABM-20435

Recombinant SARS-CoV-2 Spike S1+S2 ECD (ΔHV69-70,ΔY144,N501Y,A570D,D614G,P681H,T716I,S982A,D1118H)(His Tag)

Immobilized Human ACE2 (mFc tag)(Cat: PKSH031870) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (HV69-70 deletionY144 deletionN501YA570DD614GP681HT716IS982AD1118H)-His(PKSV030346)the EC50 of PKSV030346 is 200-800 ng/mL.Baculovirus-Insect Cells< 1.0 EU per μg of the protein as determined by the LAL method.

Recombinant SARS-CoV-2 Spike S1+S2 ECD (ΔHV69-70,ΔY144,N501Y,A570D,D614G,P681H,T716I,S982A,D1118H)(His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›

Related formats
Measured by its binding ability in a functional ELISA. Immobilized Human ACE2 (mFc tag)(Cat: PKSH031870) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H)-His(Cat: PKSV030346), the EC<sub>50</sub> of SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H)-His(Cat: PKSV030346) is 200-800 ng/mL.
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Measured by its binding ability in a functional ELISA. Immobilized Human ACE2 (mFc tag)(Cat: PKSH031870) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H)-His(Cat: PKSV030346), the EC<sub>50</sub> of SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H)-His(Cat: PKSV030346) is 200-800 ng/mL.["Immobilized Human ACE2 (mFc tag)(Cat: PKSH031870) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H)-His(PKSV030346), the EC50 of PKSV030346 is 200-800 ng/mL. ,"]

Product Description

SARS-CoV-2 Spike S1+S2 ECD (ΔHV69-70,ΔY144,N501Y,A570D,D614G,P681H,T716I,S982A,D1118H) is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.

Protein profile: reactive species/source: SARS-CoV-2; expression system: Baculovirus-Insect Cells; physical form: Lyophilized from sterile 20 mM Tris, 300 mM NaCl, 10 % glycerol, pH 8.0.<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: SARS-CoV-2 ,SARS-CoV-2 Spike S1+S2 ECD (ΔHV69-70,ΔY144,N501Y,A570D,D614G,P681H,T716I,S982A,D1118H) ,Recombinant Proteins; predicted molecular weight: 134.1 kDa.

Quality and formulation: purity: > 85 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..

Functional evidence: activity: Immobilized Human ACE2 (mFc tag)(Cat: PKSH031870) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H)-His(PKSV030346), the EC<sub>50</sub> of PKSV030346 is 200-800 ng/mL.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.

Sequence coverage: 134.1 kDa.

Database references: UniProt: YP_009724390.1 | Gene symbol: SARS-CoV-2 Spike S1+S2 ECD (ΔHV69-70,ΔY144,N501Y,A570D,D614G,P681H,T716I,S982A,D1118H).

Research Use Only.

Key Facts

Product type Recombinant Proteins› Target: SARS-CoV-2 Spike S1+S2 ECD (ΔHV69-70,ΔY144,N501Y,A570D,D614G,P681H,T716I,S982A,D1118H)
SARS-CoV-2 Spike S1+S2 ECD (ΔHV69-70,ΔY144,N501Y,A570D,D614G,P681H,T716I,S982A,D1118H)UniProt: YP_009724390.1
Target SARS-CoV-2 Spike S1+S2 ECD (ΔHV69-70,ΔY144,N501Y,A570D,D614G,P681H,T716I,S982A,D1118H)
Also known as SARS-CoV-, coronavirus s1 Protein, coronavirus s2 Protein
Species reactivity SARS-CoV-2
Purity > 85 % as determined by reducing SDS-PAGE.
Form / buffer Lyophilized from sterile 20 mM Tris, 300 mM NaCl, 10 % glycerol, pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Shelf life 12 months
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ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
BIOACTIVITYBINDINGCELL-BASEDIN VIVOELISA CAPTURE
SARS-CoV-2

Specifications

Target
SARS-CoV-2 Spike S1+S2 ECD (ΔHV69-70,ΔY144,N501Y,A570D,D614G,P681H,T716I,S982A,D1118H)
Also known as
SARS-CoV-, coronavirus s1 Protein, coronavirus s2 Protein
Purity
> 85 % as determined by reducing SDS-PAGE.
Form / buffer
Lyophilized from sterile 20 mM Tris, 300 mM NaCl, 10 % glycerol, pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Storage & Stability

Shipped at
This product is provided as lyophilized powder which is shipped with ice packs.
Short-term storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Long-term storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Aliquoting advice
Reconstitute entire vial immediately. Aliquot working stock; avoid repeated freeze-thaw.
Shelf life
12 months

Compliance & Certifications

ISO 9001:2015
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

ISO 14001:2015
ISO 14001:2015 Certified

Manufactured under ISO 14001:2015 quality management standards.

ISO 45001:2018
ISO 45001:2018 Certified

Manufactured under ISO 45001:2018 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

REACH
REACH Compliant

Substance does not appear on the SVHC candidate list.

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