Collaborator Elabscience
Recombinant ProteinsISO 9001:2015ISO 14001:2015ISO 45001:2018Collaborator Tested
ABM-20108

Recombinant Rat ICAM-1/CD54 Protein (His Tag)

Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to rat ICAM1 coated plates (12.5μg/mL100μL/well)approximately > 30% cells will adhere specificallHEK293 Cells< 1.0 EU per μg of the protein as determined by the LAL method.

Recombinant Rat ICAM-1/CD54 Protein (His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›

> 97 % as determined by reducing SDS-PAGE.
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> 97 % as determined by reducing SDS-PAGE.["Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to rat ICAM1 coated plates (12.5μg/mL, 100μL/well), approximately > 30% cells will adhere specificall ,70-75 kDa"]

Product Description

ICAM-1/CD54 is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.

Protein profile: reactive species/source: Rat; expression system: HEK293 Cells; physical form: Lyophilized from sterile PBS, pH 7.4<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Rat ,ICAM-1/CD54 ,Recombinant Proteins; predicted molecular weight: 52.7 kDa.

Quality and formulation: purity: > 97 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..

Functional evidence: activity: Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to rat ICAM1 coated plates (12.5μg/mL, 100μL/well), approximately > 30% cells will adhere specificall. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.

Sequence coverage: 52.7 kDa.

Database references: UniProt: Q00238-1 | NCBI Gene: 25464 | Gene symbol: ICAM-1/CD54.

Research Use Only.

Key Facts

Product typeRecombinant Proteins Target: ICAM-1/CD54
Collaborator Elabscience
Partner model PKSR030397_100ug
Target ICAM-1/CD54
Reactivity Rat
Purity >97% by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Shelf life 12 months
AvailabilityIn stock - ships within 2 business days
Lead time2-3 business days
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Validation Matrix

1 category
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
CategoryBioactivityBindingCell-BasedIn VivoELISA Capture
Rat

Specifications

Target
ICAM-1/CD54
Also known as
CD54, ICAM
Purity
>97% by SDS-PAGE
Form / buffer
Lyophilized

Storage & Stability

Shipped at
This product is provided as lyophilized powder which is shipped with ice packs.
Short-term storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Aliquoting advice
Reconstitute entire vial immediately. Aliquot working stock; avoid repeated freeze-thaw.
Shelf life
12 months

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001:2015

Quality management certification.

ISO
ISO 14001:2015

Environmental management certification.

ISO
ISO 45001:2018

Occupational health and safety management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

REACH
REACH

REACH compliant.

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