Recombinant Rat TNFR1/TNFRSF1A Protein (Fc Tag)
Recombinant Rat TNFR1/TNFRSF1A Protein (Fc Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›
["1. Measured by its ability to inhibit TNFα-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 ng/mL in the presence of 0.05 ng/mL of ratTNFα. 2. Immobilized mouse TNFa (80-235) at 10 μg/ml (100 μl/well) can bind rat TNFRSF1A-Fc, The EC50 of rat TNFRSF1A-Fc is 0.08-0.20 μg/ml. ,59 kDa"]Product Description
TNFR1/TNFRSF1A is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.
Protein profile: reactive species/source: Rat; expression system: HEK293 Cells; physical form: Lyophilized from sterile PBS, pH 7.4<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Rat ,TNFR1/TNFRSF1A ,Recombinant Proteins; predicted molecular weight: 47.9 kDa.
Quality and formulation: purity: > 90 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..
Functional evidence: activity: 1. Measured by its ability to inhibit TNFα-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is typically 3-30 ng/mL in the presence of 0.05 ng/mL of ratTNFα. 2. Immobilized mouse TNFa (80-235) at 10 μg/ml (100 μl/well) can bind rat TNFRSF1A-Fc, The EC<sub>50</sub> of rat TNFRSF1A-Fc is 0.08-0.20 μg/ml.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.
Sequence coverage: 47.9 kDa.
Database references: UniProt: P22934 | NCBI Gene: 25625 | Gene symbol: TNFR1/TNFRSF1A.
Research Use Only.
Key Facts
| Target | TNFR1/TNFRSF1A |
| Also known as | TNFRSF1A, Tnfr-1, Tnfr1 |
| Species reactivity | Rat |
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Form / buffer | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Shelf life | 12 months |
| BIOACTIVITY | BINDING | CELL-BASED | IN VIVO | ELISA CAPTURE | |
|---|---|---|---|---|---|
| Rat |
Specifications
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Manufactured under ISO 14001:2015 quality management standards.
Manufactured under ISO 45001:2018 quality management standards.
Not intended for diagnostic or therapeutic use.
Substance does not appear on the SVHC candidate list.