Recombinant Mouse TNF-alpha/TNFA Protein
Recombinant Mouse TNF-alpha/TNFA Protein supplied by Elabscience with collaborator-provided technical specifications. Read more ›
["Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 2-8 pg/ml. ,14 kDa"]Product Description
TNF-alpha is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.
Protein profile: reactive species/source: Mouse; expression system: E.coli; physical form: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Mouse ,TNF-alpha ,Cytokines; predicted molecular weight: 16.4 kDa.
Quality and formulation: purity: > 95 % as determined by reducing SDS-PAGE.; endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method..
Functional evidence: activity: Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 2-8 pg/ml.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.
Sequence coverage: 16.4 kDa.
Database references: UniProt: P06804 | NCBI Gene: 21926 | Gene symbol: TNF-alpha.
Research Use Only.
Key Facts
| Target | TNF-alpha |
| Also known as | Cachectin, DIF, Membrane Form, TNF-Alpha, TNF-a, TNF-alpha, TNFSF2, TNFalpha, Tnf, Tnfa, Tnfsf1a, Tnfsf2, Tumor Necrosis Factor, Tumor Necrosis Factor Ligand Superfamily Member 2 |
| Species reactivity | Mouse |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Form / buffer | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Shelf life | 12 months |
| BIOACTIVITY | BINDING | CELL-BASED | IN VIVO | ELISA CAPTURE | |
|---|---|---|---|---|---|
| Mouse |
Specifications
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Manufactured under ISO 14001:2015 quality management standards.
Manufactured under ISO 45001:2018 quality management standards.
Not intended for diagnostic or therapeutic use.
Substance does not appear on the SVHC candidate list.