Collaborator Elabscience
Recombinant ProteinsISO 9001:2015ISO 14001:2015ISO 45001:2018Collaborator Tested
ABM-19212

Recombinant Mouse CD16-2/FCGR4 Protein (His Tag)

Measured by its binding ability in a functional ELISA. Immobilized mouse FCGR4 at 10 μg/ml (100 μl/well) can bind recombinant human IgG1 (Fc). The EC50 of human IgG1 (Fc) is 0.11-0.25 μg/ml.HEK293 Cells< 1.0 EU per μg of the protein as determined by the LAL method.

Recombinant Mouse CD16-2/FCGR4 Protein (His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›

Loaded Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (OKT3) on ProA Biosensor, can bind Recombinant Mouse FCGR4 Protein, His Tag (Cat: PKSM040893) with an affinity constant of 3.43nM as determined in BLI assay (Sartorius Octet RED384)(QC tested).
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Loaded Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (OKT3) on ProA Biosensor, can bind Recombinant Mouse FCGR4 Protein, His Tag (Cat: PKSM040893) with an affinity constant of 3.43nM as determined in BLI assay (Sartorius Octet RED384)(QC tested).["Measured by its binding ability in a functional ELISA. Immobilized mouse FCGR4 at 10 μg/ml (100 μl/well) can bind recombinant human IgG1 (Fc). The EC50 of human IgG1 (Fc) is 0.11-0.25 μg/ml. ,25-35 kDa"]

Product Description

CD16-2/FCGR4 is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.

Protein profile: reactive species/source: Mouse; expression system: HEK293 Cells; physical form: Lyophilized from sterile PBS, pH 7.4<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Mouse ,CD16-2/FCGR4 ,Recombinant Proteins; predicted molecular weight: 22.4 kDa.

Quality and formulation: purity: > 95 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..

Functional evidence: activity: Measured by its binding ability in a functional ELISA. Immobilized mouse FCGR4 at 10 μg/ml (100 μl/well) can bind recombinant human IgG1 (Fc). The EC<sub>50</sub> of human IgG1 (Fc) is 0.11-0.25 μg/ml.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.

Sequence coverage: 22.4 kDa.

Database references: UniProt: NP_653142.2 | Gene symbol: CD16-2/FCGR4.

Research Use Only.

Key Facts

Product typeRecombinant Proteins Target: CD16-2/FCGR4
Collaborator Elabscience
Partner model PKSM040893_100ug
Target CD16-2/FCGR4
Reactivity Mouse
Purity >95% by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Shelf life 12 months
AvailabilityIn stock - ships within 2 business days
Lead time2-3 business days
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Validation Matrix

1 category
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
CategoryBioactivityBindingCell-BasedIn VivoELISA Capture
Mouse

Specifications

Target
CD16-2/FCGR4
Also known as
4833442P21Rik, CD16-2, FcgR4, FcgRIV, FcgammaRIV, Fcgr3a, Fcrl3, Low Affinity Immunoglobulin Gamma Fc Region Receptor IV
Purity
>95% by SDS-PAGE
Form / buffer
Lyophilized

Storage & Stability

Shipped at
This product is provided as lyophilized powder which is shipped with ice packs.
Short-term storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Aliquoting advice
Reconstitute entire vial immediately. Aliquot working stock; avoid repeated freeze-thaw.
Shelf life
12 months

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001:2015

Quality management certification.

ISO
ISO 14001:2015

Environmental management certification.

ISO
ISO 45001:2018

Occupational health and safety management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

REACH
REACH

REACH compliant.

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