Recombinant Mouse Motch A/NOTCH1 Protein (His Tag)
Recombinant Mouse Motch A/NOTCH1 Protein (His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›
["1. Immobilized human DLL4 at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC50 of biotinylated mouse NOTCH1-his is 40 ng/mL. 2. Immobilized mouse DLL4-his at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC50 of biotinylated mouse NOTCH1-his is 30 ng/mL. ,80 kDa"]Product Description
Motch A/NOTCH1 is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.
Protein profile: reactive species/source: Mouse; expression system: Baculovirus-Insect Cells; physical form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Mouse ,Motch A/NOTCH1 ,Recombinant Proteins; predicted molecular weight: 55.0 kDa.
Quality and formulation: purity: > 82 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..
Functional evidence: activity: 1. Immobilized human DLL4 at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC<sub>50</sub> of biotinylated mouse NOTCH1-his is 40 ng/mL. 2. Immobilized mouse DLL4-his at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC<sub>50</sub> of biotinylated mouse NOTCH1-his is 30 ng/mL.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.
Sequence coverage: 55.0 kDa.
Database references: UniProt: NP_032740.3 | Gene symbol: Motch A/NOTCH1.
Research Use Only.
Key Facts
| Target | Motch A/NOTCH1 |
| Also known as | Mis6, Motch A, Mt14, N1, Neurogenic locus notch homolog protein 1, Notch 1, Tan1, lin-12 |
| Species reactivity | Mouse |
| Purity | > 82 % as determined by reducing SDS-PAGE. |
| Form / buffer | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Shelf life | 12 months |
| BIOACTIVITY | BINDING | CELL-BASED | IN VIVO | ELISA CAPTURE | |
|---|---|---|---|---|---|
| Mouse |
Specifications
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Manufactured under ISO 14001:2015 quality management standards.
Manufactured under ISO 45001:2018 quality management standards.
Not intended for diagnostic or therapeutic use.
Substance does not appear on the SVHC candidate list.