Recombinant Human IL-25 protein(His Tag)
2. Measure by its ability to induce CXCL1 secretion in HT29 cells. The ED50 for this effect is <1 ng/mL.E.coli< 0.1 EU per μg of the protein as determined by the LAL method.
Recombinant Human IL-25 protein(His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›
["1. Measure by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is <5 ng/mL. 2. Measure by its ability to induce CXCL1 secretion in HT29 cells. The ED50 for this effect is <1 ng/mL. ,19 kDa"]
Product Description
IL-25 is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.
Protein profile: reactive species/source: Human; expression system: E.coli; physical form: Lyophilized from sterile PBS,pH 8.0.<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,IL-25 ,Cytokines; predicted molecular weight: 17.7 kDa.
Quality and formulation: purity: > 98 % as determined by reducing SDS-PAGE.; endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method..
Functional evidence: activity: 1. Measure by its ability to induce IL-8 secretion in human PBMCs. The ED<sub>50</sub> for this effect is <5 ng/mL. <br/>2. Measure by its ability to induce CXCL1 secretion in HT29 cells. The ED<sub>50</sub> for this effect is <1 ng/mL.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.
Sequence coverage: 17.7 kDa.
Database references: UniProt: Q9H293 | NCBI Gene: 64806 | Gene symbol: IL-25.
Research Use Only.
Key Facts
| Target | IL-25 |
| Also known as | UNQ, Interleukin, IL-17E, IL-25, Interleukin-17E, Interleukin-25, IL17E, IL25, UNQ3120, PRO10272 |
| Species reactivity | Human |
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Form / buffer | Lyophilized from sterile PBS,pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Shelf life | 12 months |
| Research area | Immunology |
| BIOACTIVITY | BINDING | CELL-BASED | IN VIVO | ELISA CAPTURE | |
|---|---|---|---|---|---|
| Human |
Specifications
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Manufactured under ISO 14001:2015 quality management standards.
Manufactured under ISO 45001:2018 quality management standards.
Not intended for diagnostic or therapeutic use.
Substance does not appear on the SVHC candidate list.