Collaborator Elabscience
Recombinant ProteinsISO 9001:2015ISO 14001:2015ISO 45001:2018Collaborator Tested
ABM-18271

Recombinant Human CD86/B7-2 Protein (Val185Ile, His Tag)

Loaded Human B7-2-His on HIS1K Biosensorcan bind Mouse CTLA-4-Fc with an affinity constant of 5.65 nM as determined in BLI assay.HEK293 Cells< 1.0 EU per μg of the protein as determined by the LAL method.

Recombinant Human CD86/B7-2 Protein (Val185Ile, His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›

Recombinant Human CD86/B7-2 Protein (Val185Ile, His Tag) - ABMIUM
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Recombinant Human CD86/B7-2 Protein (Val185Ile, His Tag) - ABMIUM["Loaded Human B7-2-His on HIS1K Biosensor, can bind Mouse CTLA-4-Fc with an affinity constant of 5.65 nM as determined in BLI assay. ,57-66 kDa"]

Product Description

CD86 is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.

Protein profile: reactive species/source: Human; expression system: HEK293 Cells; physical form: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,CD86 ,Recombinant Proteins; predicted molecular weight: 26.7 kDa.

Quality and formulation: purity: > 95 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..

Functional evidence: activity: Loaded Human B7-2-His on HIS1K Biosensor, can bind Mouse CTLA-4-Fc with an affinity constant of 5.65 nM as determined in BLI assay.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.

Sequence coverage: 26.7 kDa.

Database references: UniProt: AAH40261.1 | NCBI Gene: 942 | Gene symbol: CD86.

Research Use Only.

Key Facts

Product typeRecombinant Proteins Target: CD86
Collaborator Elabscience
Partner model PKSH033722_10ug
Target CD86
Research areasMicrobiology, immunology, Stem cells
Reactivity Human
Purity >95% by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Shelf life 12 months
AvailabilityIn stock - ships within 2 business days
Lead time2-3 business days
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Validation Matrix

1 category
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
CategoryBioactivityBindingCell-BasedIn VivoELISA Capture
Human

Specifications

Target
CD86
Also known as
LAB, ETC, FUN, CTLA-4 Counter-Receptor B, Early T-cell costimulatory molecule, CD28LG, B7-2, B7.2, B70, CD28LG2, LAB72, CD86, Activation B7-2 antigen, BU63, CTLA-4 Counter-Receptor B7.2, Early T-cell costimulatory molecule 1, ETC-1, FUN-1, T-lymphocyte activation antigen CD86, B7-2, Activation B7-2 antigen 3, B7 2, B72 antigen, B-lymphocyte activation antigen B7-2, B-lymphocyte activation antigen B7-2 2, CD28 antigen ligand 2, CD28 antigen ligand 2 2, Cd28l2, CD86 antigen, CD86 molecule, CLS1, CTLA-4 counter-receptor B7.2 2, FUN 1, Ly58, Ly-58, MB7, MB7-2, Membrane glycoprotein, MGC34413, TS/A-2
Purity
>95% by SDS-PAGE
Form / buffer
Lyophilized

Storage & Stability

Shipped at
This product is provided as lyophilized powder which is shipped with ice packs.
Short-term storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Aliquoting advice
Reconstitute entire vial immediately. Aliquot working stock; avoid repeated freeze-thaw.
Shelf life
12 months

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001:2015

Quality management certification.

ISO
ISO 14001:2015

Environmental management certification.

ISO
ISO 45001:2018

Occupational health and safety management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

REACH
REACH

REACH compliant.

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