Recombinant Human TNF beta/TNFB Protein
Recombinant Human TNF beta/TNFB Protein supplied by Elabscience with collaborator-provided technical specifications. Read more ›
["Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is <3 pg/mL. The specific activity of recombinant human TNF beta is > 3.3x 108 IU/mg. ,21 kDa"]Product Description
TNF beta is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.
Protein profile: reactive species/source: Human; expression system: E.coli; physical form: Lyophilized from sterile 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. <br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,TNF beta ,Cytokines; predicted molecular weight: 18.7 kDa.
Quality and formulation: purity: > 98 % as determined by reducing SDS-PAGE.; endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method..
Functional evidence: activity: Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED<sub>50</sub> for this effect is <3 pg/mL. The specific activity of recombinant human TNF beta is > 3.3x 10<sup>8</sup> IU/mg.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.
Sequence coverage: 18.7 kDa.
Database references: UniProt: P01374 | NCBI Gene: 4049 | Gene symbol: TNF beta.
Research Use Only.
Key Facts
| Target | TNF beta |
| Also known as | LT-Alpha, LTA, Lymphotoxin-Alpha, TNF-Beta, TNFB, TNFSF1, Tumor Necrosis Factor Ligand Superfamily Member 1 |
| Species reactivity | Human |
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Form / buffer | Lyophilized from sterile 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Shelf life | 12 months |
| Research area | Signal Transduction, Cancer, immunology, |
| BIOACTIVITY | BINDING | CELL-BASED | IN VIVO | ELISA CAPTURE | |
|---|---|---|---|---|---|
| Human |
Specifications
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Manufactured under ISO 14001:2015 quality management standards.
Manufactured under ISO 45001:2018 quality management standards.
Not intended for diagnostic or therapeutic use.
Substance does not appear on the SVHC candidate list.