Home » Recombinant Human VEGFR2/Flk-1/KDR Protein (His Tag)
Cytokines ISO 9001:2015ISO 14001:2015ISO 45001:2018ABMIUM Verified™Collaborator Tested
ABM-16801

Recombinant Human VEGFR2/Flk-1/KDR Protein (His Tag)

1. Using the Octet RED Systemthe affinity constant (Kd) of human VEGFR2-his bound to biotinylated human VEGF165 was 5.9nM. 2. Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells(HUVEC). The ED50 for this effect is typically 10-50μg/mL in the presence of 10 ng/mL recombinant human VEGF165.HEK293 Cells< 1.0 EU per μg of the protein as determined by the LAL method.

Recombinant Human VEGFR2/Flk-1/KDR Protein (His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›

Related formats
Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells(HUVEC). The ED<sub>50</sub> for this effect is typically 10-50μg/mL in the presence of 10 ng/mL recombinant human VEGF165.
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Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells(HUVEC). The ED<sub>50</sub> for this effect is typically 10-50μg/mL in the presence of 10 ng/mL recombinant human VEGF165.["1. Using the Octet RED System, the affinity constant (Kd) of human VEGFR2-his bound to biotinylated human VEGF165 was 5.9nM. 2. Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells(HUVEC). The ED50 for this effect is typically 10-50μg/mL in the presence of 10 ng/mL recombinant human VEGF165. ,120-130 kDa"]

Product Description

VEGFR2/Flk-1/KDR is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.

Protein profile: reactive species/source: Human; expression system: HEK293 Cells; physical form: Lyophilized from sterile PBS, pH 7.4<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,VEGFR2/Flk-1/KDR ,Cytokines; predicted molecular weight: 84.6 kDa.

Quality and formulation: purity: > 97 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..

Functional evidence: activity: 1. Using the Octet RED System, the affinity constant (Kd) of human VEGFR2-his bound to biotinylated human VEGF165 was 5.9nM. 2. Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells(HUVEC). The ED<sub>50</sub> for this effect is typically 10-50μg/mL in the presence of 10 ng/mL recombinant human VEGF165.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.

Sequence coverage: 84.6 kDa.

Database references: UniProt: NP_002244.1 | Gene symbol: VEGFR2/Flk-1/KDR.

Research Use Only.

Key Facts

Product type Cytokines› Target: VEGFR2/Flk-1/KDR
VEGFR2/Flk-1/KDRUniProt: NP_002244.1
Target VEGFR2/Flk-1/KDR
Also known as CD309, FLK-1, FLK1, Fetal liver kinase 1, Flk-1, KDR, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1, VEGFR, VEGFR-2, VEGFR2, Vascular endothelial growth factor receptor 2
Species reactivity Human
Purity > 97 % as determined by reducing SDS-PAGE.
Form / buffer Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Shelf life 12 months
Research area Signal Transduction, Microbiology, Cardiovascular, Cancer, metabolism, Stem cells
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1
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
BIOACTIVITYBINDINGCELL-BASEDIN VIVOELISA CAPTURE
Human

Specifications

Target
VEGFR2/Flk-1/KDR
Also known as
CD309, FLK-1, FLK1, Fetal liver kinase 1, Flk-1, KDR, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1, VEGFR, VEGFR-2, VEGFR2, Vascular endothelial growth factor receptor 2
Purity
> 97 % as determined by reducing SDS-PAGE.
Form / buffer
Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Storage & Stability

Shipped at
This product is provided as lyophilized powder which is shipped with ice packs.
Short-term storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Long-term storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Aliquoting advice
Reconstitute entire vial immediately. Aliquot working stock; avoid repeated freeze-thaw.
Shelf life
12 months

Compliance & Certifications

ISO 9001:2015
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

ISO 14001:2015
ISO 14001:2015 Certified

Manufactured under ISO 14001:2015 quality management standards.

ISO 45001:2018
ISO 45001:2018 Certified

Manufactured under ISO 45001:2018 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

REACH
REACH Compliant

Substance does not appear on the SVHC candidate list.

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