Recombinant Human CSF1R/CD115 Protein (aa 1-512, His Tag)
2. Measured by its ability to inhibit the human CSF-induced proliferation of M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 6-30 μg/mL in the presence of 3 ng/ml Recombinant Human M-CSF.HEK293 Cells< 1.0 EU per μg of the protein as determined by the LAL method.
Recombinant Human CSF1R/CD115 Protein (aa 1-512, His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›
["1. Immobilized human CSF1R at 10 μg/mL (100 μl/well) can bind biotinylated human CSF-1, The EC50 of biotinylated human CSF-1 is 8 ng/mL. 2. Measured by its ability to inhibit the human CSF-induced proliferation of M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 6-30 μg/mL in the presence of 3 ng/ml Recombinant Human M-CSF. ,85-95 kDa"]
Product Description
CSF1R/CD115 is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.
Protein profile: reactive species/source: Human; expression system: HEK293 Cells; physical form: Lyophilized from sterile PBS, pH 7.4<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,CSF1R/CD115 ,Recombinant Proteins; predicted molecular weight: 56.0 kDa.
Quality and formulation: purity: > 98 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..
Functional evidence: activity: 1. Immobilized human CSF1R at 10 μg/mL (100 μl/well) can bind biotinylated human CSF-1, The EC<sub>50</sub> of biotinylated human CSF-1 is 8 ng/mL. <br/>2. Measured by its ability to inhibit the human CSF-induced proliferation of M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED<sub>50</sub> for this effect is typically 6-30 μg/mL in the presence of 3 ng/ml Recombinant Human M-CSF.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.
Sequence coverage: 56.0 kDa.
Database references: UniProt: NP_005202.2 | NCBI Gene: 1436 | Gene symbol: CSF1R/CD115.
Research Use Only.
Key Facts
| Target | CSF1R/CD115 |
| Also known as | C-FMS, CD115, CSF-1 receptor, CSF-1-R, CSF-1R, CSF1R, FIM2, FMS, HDLS, M-CSF-R, MCSF Receptor , Macrophage colony-stimulating factor 1 receptor, Proto-oncogene c-Fms |
| Species reactivity | Human |
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Form / buffer | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Shelf life | 12 months |
| Research area | Signal Transduction, Cardiovascular, immunology, |
| BIOACTIVITY | BINDING | CELL-BASED | IN VIVO | ELISA CAPTURE | |
|---|---|---|---|---|---|
| Human |
Specifications
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Manufactured under ISO 14001:2015 quality management standards.
Manufactured under ISO 45001:2018 quality management standards.
Not intended for diagnostic or therapeutic use.
Substance does not appear on the SVHC candidate list.