Recombinant Human IL2Ra/CD25 Protein (His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›
["1. Using the Octet RED System, the affinity constant (Kd) of Human IL2RA-his bound to Human IL2 was 35nM. 2. Measured by its ability to inhibit IL2-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 30 ng/mL of recombinant human IL-2. The ED50 for this effect is 0.15-0.75μg/mL. ,44 kDa"]IL2Ra/CD25 is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.
Protein profile: reactive species/source: Human; expression system: HEK293 Cells; physical form: Lyophilized from sterile PBS, pH 7.4<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,IL2Ra/CD25 ,Recombinant Proteins; predicted molecular weight: 23.3 kDa.
Quality and formulation: purity: > 97 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..
Functional evidence: activity: 1. Using the Octet RED System, the affinity constant (Kd) of Human IL2RA-his bound to Human IL2 was 35nM. 2. Measured by its ability to inhibit IL2-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 30 ng/mL of recombinant human IL-2. The ED<sub>50</sub> for this effect is 0.15-0.75μg/mL.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.
Sequence coverage: 23.3 kDa.
Database references: UniProt: NP_000408.1 | NCBI Gene: 3559 | Gene symbol: IL2Ra/CD25.
Research Use Only.
| Collaborator | Elabscience |
| Partner model | PKSH031814_100ug |
| Target | IL2Ra/CD25 |
| Research areas | immunology |
| Reactivity | Human |
| Purity | >97% by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Shelf life | 12 months |
| Availability | In stock - ships within 2 business days |
| Lead time | 2-3 business days |
| Category | Bioactivity | Binding | Cell-Based | In Vivo | ELISA Capture |
|---|---|---|---|---|---|
| Human |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
Environmental management certification.
Occupational health and safety management certification.
For research applications. Not for diagnostic use.
REACH compliant.
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