Home » Recombinant Human IL2Ra/CD25 Protein (His Tag)
Recombinant Proteins ISO 9001:2015ISO 14001:2015ISO 45001:2018ABMIUM Verified™Collaborator Tested
ABM-16711

Recombinant Human IL2Ra/CD25 Protein (His Tag)

1. Using the Octet RED Systemthe affinity constant (Kd) of Human IL2RA-his bound to Human IL2 was 35nM. 2. Measured by its ability to inhibit IL2-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 30 ng/mL of recombinant human IL-2. The ED50 for this effect is 0.15-0.75μg/mL.HEK293 Cells< 1.0 EU per μg of the protein as determined by the LAL method.

Recombinant Human IL2Ra/CD25 Protein (His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›

Related formats
Measured by its ability to inhibit IL2-induced proliferation of M07e cells. The ED<sub>50</sub> for this effect is 0.2-1.2 μg/mL in the presence of 30ng/mL of recombinant human IL2.
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Measured by its ability to inhibit IL2-induced proliferation of M07e cells. The ED<sub>50</sub> for this effect is 0.2-1.2 μg/mL in the presence of 30ng/mL of recombinant human IL2.["1. Using the Octet RED System, the affinity constant (Kd) of Human IL2RA-his bound to Human IL2 was 35nM. 2. Measured by its ability to inhibit IL2-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 30 ng/mL of recombinant human IL-2. The ED50 for this effect is 0.15-0.75μg/mL. ,44 kDa"]

Product Description

IL2Ra/CD25 is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.

Protein profile: reactive species/source: Human; expression system: HEK293 Cells; physical form: Lyophilized from sterile PBS, pH 7.4<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,IL2Ra/CD25 ,Recombinant Proteins; predicted molecular weight: 23.3 kDa.

Quality and formulation: purity: > 97 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..

Functional evidence: activity: 1. Using the Octet RED System, the affinity constant (Kd) of Human IL2RA-his bound to Human IL2 was 35nM. 2. Measured by its ability to inhibit IL2-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 30 ng/mL of recombinant human IL-2. The ED<sub>50</sub> for this effect is 0.15-0.75μg/mL.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.

Sequence coverage: 23.3 kDa.

Database references: UniProt: NP_000408.1 | NCBI Gene: 3559 | Gene symbol: IL2Ra/CD25.

Research Use Only.

Key Facts

Product type Recombinant Proteins› Target: IL2Ra/CD25
IL2Ra/CD25UniProt: NP_000408.1Gene ID: 3559
Target IL2Ra/CD25
Also known as IMD, IDDM, IL2RA, CD25, IDDM10, IL2R, IMD41, TCGFR, p55, IL-2 receptor subunit alpha, IL-2R subunit alpha, IL2-RA, IL-2-RA, IL-2Rα, Interleukin-2 receptor subunit alpha, TAC antigen, Tac, p55, IL-2R&, alpha, alpha chain, CD25 antigen, IL 2 receptor alpha subunit, IL2 RA, Interleukin 2 receptor, Interleukin 2 receptor alpha, Interleukin 2 receptor alpha chain, t-cell growth factor receptor
Species reactivity Human
Purity > 97 % as determined by reducing SDS-PAGE.
Form / buffer Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Shelf life 12 months
Research area immunology
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1
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
BIOACTIVITYBINDINGCELL-BASEDIN VIVOELISA CAPTURE
Human

Specifications

Target
IL2Ra/CD25
Also known as
IMD, IDDM, IL2RA, CD25, IDDM10, IL2R, IMD41, TCGFR, p55, IL-2 receptor subunit alpha, IL-2R subunit alpha, IL2-RA, IL-2-RA, IL-2Rα, Interleukin-2 receptor subunit alpha, TAC antigen, Tac, p55, IL-2R&, alpha, alpha chain, CD25 antigen, IL 2 receptor alpha subunit, IL2 RA, Interleukin 2 receptor, Interleukin 2 receptor alpha, Interleukin 2 receptor alpha chain, t-cell growth factor receptor
Purity
> 97 % as determined by reducing SDS-PAGE.
Form / buffer
Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Storage & Stability

Shipped at
This product is provided as lyophilized powder which is shipped with ice packs.
Short-term storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Long-term storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Aliquoting advice
Reconstitute entire vial immediately. Aliquot working stock; avoid repeated freeze-thaw.
Shelf life
12 months

Compliance & Certifications

ISO 9001:2015
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

ISO 14001:2015
ISO 14001:2015 Certified

Manufactured under ISO 14001:2015 quality management standards.

ISO 45001:2018
ISO 45001:2018 Certified

Manufactured under ISO 45001:2018 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

REACH
REACH Compliant

Substance does not appear on the SVHC candidate list.

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