Recombinant Proteins ISO 9001:2015ISO 14001:2015ISO 45001:2018ABMIUM Verified™Collaborator Tested
ABM-16704

Recombinant Human DKK1/Dkk-1 Protein (His Tag)

Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.1-0.4 μg/ml in the presence of 10 ng/mL of mouse Wnt3a.HEK293 Cells< 1.0 EU per μg of the protein as determined by the LAL method.

Recombinant Human DKK1/Dkk-1 Protein (His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›

Related formats
Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED<sub>50</sub> for this effect is approximately 0.1-0.4 μg/ml in the presence of 10 ng/mL of mouse Wnt3a.
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Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED<sub>50</sub> for this effect is approximately 0.1-0.4 μg/ml in the presence of 10 ng/mL of mouse Wnt3a.["Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.1-0.4 μg/ml in the presence of 10 ng/mL of mouse Wnt3a. ,42 kDa"]

Product Description

DKK1/Dkk-1 is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.

Protein profile: reactive species/source: Human; expression system: HEK293 Cells; physical form: Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.; tag: Human ,DKK1/Dkk-1 ,Recombinant Proteins; predicted molecular weight: 27.2 kDa.

Quality and formulation: purity: > 95 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..

Functional evidence: activity: Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED<sub>50</sub> for this effect is approximately 0.1-0.4 μg/ml in the presence of 10 ng/mL of mouse Wnt3a.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.

Sequence coverage: 27.2 kDa.

Database references: UniProt: NP_036374.1 | Gene symbol: DKK1/Dkk-1.

Research Use Only.

Key Facts

Product type Recombinant Proteins Target: DKK1/Dkk-1
Target DKK1/Dkk-1
Also known as Dickkopf-1, Dickkopf-related protein 1, Dkk-1, SK
Species reactivity Human
Purity > 95 % as determined by reducing SDS-PAGE.
Form / buffer Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.
Shelf life 12 months
Research area Cardiovascular, Developmental Biology, immunology, Stem cells
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1
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
BIOACTIVITYBINDINGCELL-BASEDIN VIVOELISA CAPTURE
Human

Specifications

Target
DKK1/Dkk-1
Also known as
Dickkopf-1, Dickkopf-related protein 1, Dkk-1, SK
Purity
> 95 % as determined by reducing SDS-PAGE.
Form / buffer
Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.

Storage & Stability

Shipped at
This product is provided as lyophilized powder which is shipped with ice packs.
Short-term storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Long-term storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Aliquoting advice
Reconstitute entire vial immediately. Aliquot working stock; avoid repeated freeze-thaw.
Shelf life
12 months

Compliance & Certifications

ISO 9001:2015
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

ISO 14001:2015
ISO 14001:2015 Certified

Manufactured under ISO 14001:2015 quality management standards.

ISO 45001:2018
ISO 45001:2018 Certified

Manufactured under ISO 45001:2018 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

REACH
REACH Compliant

Substance does not appear on the SVHC candidate list.

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