Recombinant Human Noggin/NOG Protein (aa 1-232, Fc Tag)
Recombinant Human Noggin/NOG Protein (aa 1-232, Fc Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›
["1. Measured by its ability to inhibit BMP2-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 1. 5-2.0 μg /mL in the presence of 0.25-0.5 μg/mL of BMP-2. 2. Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 0.1-0.6 μg/mL in the presence of 50 ng/mL of hBMP4. ,58-62 kDa"]Product Description
Noggin/NOG is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.
Protein profile: reactive species/source: Human; expression system: HEK293 Cells; physical form: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,Noggin/NOG ,Recombinant Proteins; predicted molecular weight: 49.8 kDa.
Quality and formulation: purity: > 95 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..
Functional evidence: activity: 1. Measured by its ability to inhibit BMP2-induced alkaline phosphatase production by MC3T3-E1 cells. The ED<sub>50</sub> for this effect is typically 1. 5-2.0 μg /mL in the presence of 0.25-0.5 μg/mL of BMP-2. 2. Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3-E1 cells. The ED<sub>50</sub> for this effect is typically 0.1-0.6 μg/mL in the presence of 50 ng/mL of hBMP4.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.
Sequence coverage: 49.8 kDa.
Database references: UniProt: NP_005441.1 | NCBI Gene: 9241 | Gene symbol: Noggin/NOG.
Research Use Only.
Key Facts
| Target | Noggin/NOG |
| Also known as | Noggin, SYM1, SYNS1 |
| Species reactivity | Human |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Form / buffer | Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Shelf life | 12 months |
| Research area | Developmental Biology, Stem cells |
| BIOACTIVITY | BINDING | CELL-BASED | IN VIVO | ELISA CAPTURE | |
|---|---|---|---|---|---|
| Human |
Specifications
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Manufactured under ISO 14001:2015 quality management standards.
Manufactured under ISO 45001:2018 quality management standards.
Not intended for diagnostic or therapeutic use.
Substance does not appear on the SVHC candidate list.