Recombinant Human SerpinA1/A1AT Protein (His Tag)
Recombinant Human SerpinA1/A1AT Protein (His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›
["Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog#27114). The IC50 value is < 3.0 nM, as measured in 100μL reaction mixture containing 1. 25 ng trypsin (Sigma, Catalog#T1426), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, pH 7.5. ,55-60 kDa"]Product Description
SerpinA1/A1AT is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.
Protein profile: reactive species/source: Human; expression system: HEK293 Cells; physical form: Lyophilized from sterile PBS, pH 7.4<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,SerpinA1/A1AT ,Recombinant Proteins; predicted molecular weight: 45.7 kDa.
Quality and formulation: purity: > 97 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..
Functional evidence: activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog#27114). The IC50 value is < 3.0 nM, as measured in 100μL reaction mixture containing 1. 25 ng trypsin (Sigma, Catalog#T1426), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, pH 7.5.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.
Sequence coverage: 45.7 kDa.
Database references: UniProt: NP_000286.3 | NCBI Gene: 5265 | Gene symbol: SerpinA1/A1AT.
Research Use Only.
Key Facts
| Target | SerpinA1/A1AT |
| Also known as | A1A, A1AT, AAT, Alpha-1 Protease Inhibitor, Alpha-1-Antiproteinase, Alpha-1-Antitrypsin, MGC23330, MGC9222, PI, PI1, PRO2275, SERPINA1, Serpin A1, alpha1AT |
| Species reactivity | Human |
| Purity | > 97 % as determined by reducing SDS-PAGE. |
| Form / buffer | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Shelf life | 12 months |
| Research area | Cardiovascular, Cancer, metabolism |
| BIOACTIVITY | BINDING | CELL-BASED | IN VIVO | ELISA CAPTURE | |
|---|---|---|---|---|---|
| Human |
Specifications
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Manufactured under ISO 14001:2015 quality management standards.
Manufactured under ISO 45001:2018 quality management standards.
Not intended for diagnostic or therapeutic use.
Substance does not appear on the SVHC candidate list.