Recombinant Human HMGB1/HMG1 Protein (His Tag)
Recombinant Human HMGB1/HMG1 Protein (His Tag) supplied by Elabscience with collaborator-provided technical specifications. Read more ›
["1. Immobilized human HMGB at 2 μg/ml (100 μl/well) can bind human AGER. The EC50 of human AGER is 0.27 μg/ml. 2. Measured by its ability to induce TNF-alpha secretion by RAW 264. 7 mouse monocyte/macrophage cells. The EC50 for this effect is typically 20-100μg/ml. ,30-34 kDa"]Product Description
HMGB1/HMG1 is the stated recombinant protein target for this Elabscience product. The listing uses collaborator-supplied specifications to support research-use-only protein selection through ABMIUM.
Protein profile: reactive species/source: Human; expression system: HEK293 Cells; physical form: Lyophilized from sterile PBS, pH 7.4<br/>Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.<br/>Please refer to the specific buffer information in the printed manual.; tag: Human ,HMGB1/HMG1 ,Recombinant Proteins; predicted molecular weight: 26.3 kDa.
Quality and formulation: purity: > 93 % as determined by reducing SDS-PAGE.; endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method..
Functional evidence: activity: 1. Immobilized human HMGB at 2 μg/ml (100 μl/well) can bind human AGER. The EC<sub>50</sub> of human AGER is 0.27 μg/ml. 2. Measured by its ability to induce TNF-alpha secretion by RAW 264. 7 mouse monocyte/macrophage cells. The EC<sub>50</sub> for this effect is typically 20-100μg/ml.. In the ABMIUM matrix, collaborator-supplied functional evidence is marked C; unsupported application columns remain N.
Sequence coverage: 26.3 kDa.
Database references: UniProt: NP_002119.1 | NCBI Gene: 3146 | Gene symbol: HMGB1/HMG1.
Research Use Only.
Key Facts
| Target | HMGB1/HMG1 |
| Also known as | HMG-1, HMG1, HMG3, SBP-1 |
| Species reactivity | Human |
| Purity | > 93 % as determined by reducing SDS-PAGE. |
| Form / buffer | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Shelf life | 12 months |
| Research area | epigenetics and nuclear signal |
| BIOACTIVITY | BINDING | CELL-BASED | IN VIVO | ELISA CAPTURE | |
|---|---|---|---|---|---|
| Human |
Specifications
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Manufactured under ISO 14001:2015 quality management standards.
Manufactured under ISO 45001:2018 quality management standards.
Not intended for diagnostic or therapeutic use.
Substance does not appear on the SVHC candidate list.